Identification of Lactobacillus from koumiss by conventional and molecular methods |
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| Authors: | Junguo Wang Xia Chen Wenjun Liu Mei Yang Airidengcaicike Heping Zhang |
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| Affiliation: | (1) Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Huhhot, 010018, People’s Republic of China |
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| Abstract: | Lactobacilli are considered to be one of the most important potential probiotics in the dairy industry. Twelve strains of Lactobacillus were isolated from home-made koumiss samples, a traditionally fermented mare milk in China. The isolates were identified
based on physiological and biochemical characteristics and analysis of 16S RNA sequences. They were proven to be Lactobacillus helveticus, Lactobacillus fermentum,
Lactobacillus casei and Lactobacillus plantarum. The results demonstrated that both methods were essential to identify an isolate accurately. |
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| Keywords: | Lactobacilli Koumiss Identification 16S rRNA sequencing analysis |
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