| 基质辅助激光解吸电离飞行时间质谱法分析核糖核酸酶 |
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| 引用本文： | 季怡萍,张红明. 基质辅助激光解吸电离飞行时间质谱法分析核糖核酸酶[J]. 现代仪器, 2007, 13(6): 14-15 |
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| 作者姓名： | 季怡萍 张红明 |
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| 作者单位： | 中国科学院长春应用化学研究所,吉林长春,130022 |
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| 摘 要： | 应用基质辅助激光解吸电离飞行时间质谱法(MALD I-TOF-MS)成功地对核糖核酸酶相对分子量进行测定。探讨三种不同基质对其分析结果的影响,实验中发现α-氰基-4-羟基肉桂酸是适宜的基质,该方法是传统生物方法无法比拟的。
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| 关 键 词： | 核糖核酸酶 质谱 基质 |
Characterization of ribonuclease using matrix-assisted laser desorption ionization time-of-flight mass spectrometry |
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| Ji Yiping,Zhang Hongming. Characterization of ribonuclease using matrix-assisted laser desorption ionization time-of-flight mass spectrometry[J]. Modern Instruments, 2007, 13(6): 14-15 |
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| Authors: | Ji Yiping Zhang Hongming |
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| Abstract: | Relative molecular weight of ribonuclease was successfully determined by matrix-assisted laser desorption ionization time-of-flight mass spectrometry(MALDI-TOF-MS) technique.The effect of three kinds of matrix on the analytical result was investigated.The α-cyano-4-hydroxycinnamic acid was found to be the suitable matrix and this technique was far better than conventional biological method. |
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| Keywords: | Ribonuclease Mass spectrometry Matrix |
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