| 分光光度法测定黄花草木樨中总黄酮含量 |
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| 引用本文： | 汤春妮,闫晓前,马喜锋,张军科.分光光度法测定黄花草木樨中总黄酮含量[J].湖北化工,2012(1):92-94. |
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| 作者姓名： | 汤春妮 闫晓前 马喜锋 张军科 |
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| 作者单位： | 陕西国防工业职业技术学院,陕西西安710302 |
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| 基金项目： | 陕西国防工业职业技术学院研究与开发项目（Gfy11-08） |
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| 摘 要： | 采用分光光度法测定了黄花草木樨中总黄酮的含量。以芦丁为对照品,NaNO2-Al（NO3）3-NaOH体系显色,在波长510nm处对样品中的总黄酮进行含量测定。结果表明,总黄酮在10～50μg.mL-1（R=0.9992）范围内线性关系良好,平均回收率为99.19%（RSD=0.79%,n=9）,测得样品中总黄酮平均含量为1.44%。该方法操作简便、准确,具有很好的重现性和稳定性,适用于黄花草木樨中总黄酮含量的测定。
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| 关 键 词： | 黄花草木樨 总黄酮 分光光度法 含量 测定 |
Determination of Total Flavonoids Content in Melilotus by Spectrophotometry |
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| TANG Chun-ni,YAN Xiao-qian,MA Xi-feng,ZHANG Jun-ke.Determination of Total Flavonoids Content in Melilotus by Spectrophotometry[J].Hubei Chemical Industry,2012(1):92-94. |
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| Authors: | TANG Chun-ni YAN Xiao-qian MA Xi-feng ZHANG Jun-ke |
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| Affiliation: | (Shanxi Institute of Technology,Xi′an 710302,China) |
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| Abstract: | The content of total flavonoids in Melilotus was determined by spectrophotometry.Rutin was used as the standard sample and NaNO2-Al（NO3）3-NaOH was used to compose the chromogenic system.The detection wavelength was set at 510 nm.A good linear relationship was observed in the range of 10~50 μg·mL-1（R=0.9992） and the average recovery rate was 99.19% with a relative standard deviation of 0.79%（n=9）.The average content of total flavonoids in Melilotus was 1.44%.This method is simple,reliable,accurate and rapid with good reproducibility and stability.It is a suitable way for determination of total flavonoids in Melilotus. |
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| Keywords: | Melilotus total flavonoids spectrophotometry content determination |
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