氮浓度对异养小球藻生长及蛋白质含量的影响

为了提高异养小球藻蛋白质含量低的问题,研究了异养小球藻生长及蛋白质含量和氮浓度的关系,探究了分阶段调控氮浓度对异养小球藻生长及蛋白质含量的影响。结果表明,氮浓度在3～15mmol/L范围内,小球藻生物量及蛋白质含量随氮浓度增加而增加,生物量从0.91g/L提高到了3.02g/L,蛋白质含量从26.1%提高到了37.4%。分阶段培养小球藻,首先在低氮条件下培养至指数期前期,然后转移至高氮浓度下培养,生物量达3.04g/L,且蛋白质含量提高至53.8%,与自养培养条件下蛋白质含量相当。

Effect of nitrogen concentrations on growth and protein content of heterotrophic microalgae

In order to improve the problem of low protein content of heterotrophic Chlorella vulgaris,the effects of nitrogen concentrations on growth and protein content of Chlorella vulgaris were studied.The regulations of nitrogen concentrations in different phases on the growth and protein content of Chlorella vulgaris were also studied.The results showed that protein content of Chlorella vulgaris was increased from 26.1% to 37.4% with the increase of initial nitrogen concentration from 12 to 15mmol/L.The control strategy was that firstly let Chlorella vulgaris grow under low nitrogen concentration to its exponential phase,and then transfered it to high nitrogen concentration.So we could obtaine both high biomass 3.04g/L and high protein content of 53.8%,which reaches the protein level of Chlorella vulgaris in autotrophic culture.