响应面优化虾头壳酶促水解清除DPPH自由基活性的研究

以碱性蛋白酶水解虾头壳蛋白质,在影响水解条件单因素研究基础上,采用响应面方法中心组合设计,建立以DPPH自由基清除率为指标的水解模型,优化得出最佳酶解条件,并测定水解液中多肽的相对分子质量分布。结果表明,最佳酶解条件为pH7.2、温度50.5℃、加酶量415U/g、时间120min。在此条件下水解,水解度达21.8%,DPPH自由基清除率为66.9%,平均肽段长度为4.6,酶解液中大部分肽的相对分子量低于6500u。

Study on response surface methodology to optimize the DPPH radical scavenging activity of shrimp enzymatic hydrolysate products

The purpose was using alkaline protease to optimize enzymatic hydrolysis parameters to produce peptides with high DPPH(2,2-diphenyl-1-picryl hydrazyl) radical scavenging activity(DSA) from shrimp processing by-products.A central composite design(CCD) part of response surface analysis(RSM) was used as an experimental design based on the single-factor to achieve the highest scavenging activity.The results showed that the optimal enzymatic hydrolysis conditions were temperature 50.5℃,time duration 120min,pH7.2,enzyme concentration 415U/g.The degree of hydrolysis(DH),DSA,the average length of peptides and average relative molecular weight under this condition was 21.8%,66.9%,4.6 and 6500u,respectively.