超声热浸-紫外分光光度法测定蜂巢蜜中总黄酮含量

建立了一种快速提取测定蜂巢蜜中总黄酮含量的方法—超声热浸紫外分光光度法。蜂巢蜜、蜂胶、蜂皇浆、椴树蜜的超声热浸提取液用聚酰胺柱层析去除杂质干扰后,用分光光度法在360nm波长处测定总黄酮含量。此方法回收率均在90%以上,变异系数均低于2%,具有良好的准确度。与传统方法相比,该方法具有试剂用量少,操作简单等优点,蜂巢蜜中总黄酮含量为14.97mg/100g,虽仅为蜂胶产品总黄酮含量的0.87%,但与其它蜂产品进行比较,总黄酮含量至少高出蜂王浆、洋槐蜜、椴树蜜一倍。蜂巢蜜作为一种新面市的保健食品,在黄酮类活性物质含量上表现出更高的保健价值。

Ultrasonic sound hot extract-ultraviolet spectrophotometric determination of total flavone in comb honey

Ultrasonic sound hot extract coupled with ultraviolet spectrophotometric was used to determinatate the total flavone in comb honey.Comb honey,propolis,royal jelly and linden honey were treated with polyamind column chromatography first to remove the impurities,then treated with methanol extract and ultrasonic sound hot extract.The detection method of total flavone was spectrophotometric method performed at 360nm.The mean spike recovery rates of the established method for total flavone in all samples were above 90% with a coefficient of variation of less than 2%.This method displayed excellent precision,had many advantages over the conventional method,such as less reagent consumption and ease of operation.The content of total flavone in comb honey was 14.97mg/100g,It was only 0.87% of that of propolis,but compared with some bee products,the content was nearly doubled.As a new healthy food,comb honey had digher value on total flavone.