采用原子力显微镜分析乳酸链球菌素结构的研究

目的:采用原子力显微镜对乳酸链球菌素的分子形貌进行观测,为研究其抑菌机理提供有力的科学依据和可靠、直观的实验方法。方法:从川西高原牧民自然发酵酸奶中分离筛选乳酸链球菌素产生菌,利用其发酵获得产乳酸链球菌素效价高的产生菌C201,用原子力显微镜观察其分子形貌。结果:乳酸链球菌素分子在溶液中呈树状的不规则"峰"状聚集体,带有很多侧链分支,并且肽键之间形成稳定的螺旋结构。结论:原子力显微镜能直观地表征乳酸链球菌素的分子结构,是对研究乳酸链球菌素的抑菌机理在形态学上的完善。

Study on structure of Nisin using atomic force microscope analysis

Objective:The molecules morphology of Nisin were observed using AFM,and it can provide the scientific basis and reliable,intuitive experimental methods for antibacterial mechanism of Nisin.Method:The Nisin producing strains were isolated from natural fermented yogurt of the herdsman of western Sichuan plateau by purification.A high titer Nisin producing strain C201 was obtained by applied these strains to ferment,and observed its molecular morphology using atomic force microscope analysis.Results:The molecules of Nisin could aggregate to form micelles with dendritic irregular peak shapes in solution in the form of large numbers of side chain branching,and the peptide bond that form between the base pairs along a DNA or RNA molecule stabilized the helical structure.Conclusion:The molecular structure of Nisin could characterized directly by atomic force microscopy,so it is a new and scientific analytical method to study the antibacterial mechanism of Nisin in the morphological.