| 不同对虾中多酚氧化酶的提取比较及在虾体的分布研究 |
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| 引用本文： | 吴亮亮,杨会成,廖妙飞,钟明杰,郝云彬,郑斌.不同对虾中多酚氧化酶的提取比较及在虾体的分布研究[J].食品工业科技,2012,33(7):55-57,62. |
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| 作者姓名： | 吴亮亮 杨会成 廖妙飞 钟明杰 郝云彬 郑斌 |
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| 作者单位： | 1. 浙江海洋学院食品医药学院,浙江舟山,316000
2. 浙江省海洋开发研究院,浙江舟山,316100 |
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| 基金项目： | 国家国际科技合作专项(2010DFB33030);浙江省重点科技创新团队项目(2011R09031);浙江省优先主题重点农业项目(2009C12008);“十二五”国家科技支撑计划项目子课题(2012BAD29B06) |
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| 摘 要： | 用丙酮法和匀浆浸提法分别对南美白对虾和中华管鞭虾进行多酚氧化酶的提取,结果表明,在同样的提取条件下,中华管鞭虾的酶活大于南美白对虾,匀浆浸提法提取的酶活比丙酮法高1.5倍;同时对中华管鞭虾的提取条件料液比、缓冲液pH、浸提时间、水浴温度做了研究,通过正交实验得到当料液比1∶4,缓冲液pH8,浸提时间3h,水浴温度45℃条件下提取的酶具有最大的活性;进一步研究中华管鞭虾的多酚氧化酶在虾体中的分布情况,得出虾头部位多酚氧化酶含量较高,虾尾和虾身含量较低。
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| 关 键 词： | 对虾 多酚氧化酶 酶活力 酶分布 |
Study on extraction of polyphenoloxidase and enzyme distribution on different shrimps |
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| WU Liang-liang,YANG Hui-cheng,LIAO Miao-fei,ZHONG Ming-jie,HAO Yun-bin,ZHENG Bin.Study on extraction of polyphenoloxidase and enzyme distribution on different shrimps[J].Science and Technology of Food Industry,2012,33(7):55-57,62. |
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| Authors: | WU Liang-liang YANG Hui-cheng LIAO Miao-fei ZHONG Ming-jie HAO Yun-bin ZHENG Bin |
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| Affiliation: | 1.School of Food and Pharmacy & Medical School,Zhejiang Ocean University,Zhoushan 316000,China;2.Zhejiang Marine Development Research Institute,Zhoushan 316100,China) |
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| Abstract: | Extraction of polyphenoloxidase from penaeus vannamei and solenocera crassicornis were studied using two methods,acetone method and homogenate method.It was found that the activity of polyphenoloxidase in solenocera crassicornis was higher than that in penaeus vannamei under the same extraction condition.The enzyme activity extracted by homogenate method was 1.5 times higher than acetone method.Orthogonal test result showed that the optimal extraction conditions were as follows:Solid to solvent ratio 1∶4,pH8.0,extraction time 3h,extraction temperature 45℃.And the distributed rule of polyphenoloxidase in solenocera crassicornis was studied.The results indicated that polyphenoloxidase activity was higher in the part of prawn’s head than that in the part of body and trail. |
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| Keywords: | shrimp polyphenoloxidase enzyme activity enzyme distribution |
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