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| 超声强化提取山黄皮果总黄酮及其抗氧化活性的研究 | |
| 引用本文： | 李昌宝,游向荣,孙健,李丽,张娥珍,王振兴,邱文武,王文林.超声强化提取山黄皮果总黄酮及其抗氧化活性的研究[J].食品工业科技,2012,33(5):265-268. |
| 作者姓名： | 李昌宝 游向荣 孙健 李丽 张娥珍 王振兴 邱文武 王文林 |
| 作者单位： | 1. 广西农业科学院农产品加工研究所,广西南宁530007／广西作物遗传改良生物技术重点实验室,广西南宁530007 2. 广西农业科学院农产品加工研究所,广西南宁,530007 3. 广西亚热带作物研究所试验站,广西龙州,532415 |
| 基金项目： | 广西农业科学院科技发展基金(2011JM21);广西农业科学院科技发展基金(201006) |
| 摘 要： | 采用超声强化提取山黄皮果中的总黄酮,通过正交实验方法确定山黄皮果总黄酮优化的提取工艺,并测定其对1,1-二苯基-2-三硝基苯肼自由基(DPPH.)和羟自由基(.OH)的清除效果。结果发现,在本实验条件下,超声强化提取山黄皮果总黄酮的优化提取工艺条件为提取温度70℃、液料比40:1(v/m)、乙醇浓度60%(v/v)、提取时间60min;在优化条件下,山黄皮果总黄酮得率为3.82%±0.02%;山黄皮果总黄酮对DPPH.和.OH均有较强的清除能力。研究可为山黄皮果开发利用提供参考。 |
| 关 键 词： | 山黄皮果 总黄酮 超声波提取 抗氧化活性 |
Study on ultrasonic extraction of flavonoids from Clausena indica(Datz).Oliv.and its antioxidative activity |
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| LI Chang-bao,YOU Xiang-rong,SUN Jian,LI Li,ZHANG E-zhen,WANG Zhen-xing,QIU Wen-wu,WANG Wen-lin.Study on ultrasonic extraction of flavonoids from Clausena indica(Datz).Oliv.and its antioxidative activity[J].Science and Technology of Food Industry,2012,33(5):265-268. | |
| Authors: | LI Chang-bao YOU Xiang-rong SUN Jian LI Li ZHANG E-zhen WANG Zhen-xing QIU Wen-wu WANG Wen-lin |
| Affiliation: | 1.Institute of Agro-food Science & Technology,Guangxi Academy of Agricultrual Sciences,Nanning 530007,China;2.Guangxi Crop Genetic Improvement Laboratory,Nanning 530007,China;3.Guangxi Subtropical Crops Research Institute Experimental Station,Longzhou 532415,China) |
| Abstract: | The optimum conditions for extracting flavonoids in Clausena indica(Datz).Oliv by ultrasonic were determined by using the orthogonal experimental design.Meanwhile,the flavonoids’ effect on removal of ·OH and DPPH· was investigated.The result showed that the optimal technological parameters were as follows:the extracting temperature was 70℃,the ratio of solvent and material was 40:1(v/m),the concentration of solvent was 60%(v/v),and the extracting time was 60min.Under the optimum conditions,the total extracting rate was as high as 3.82%±0.02%.The flavonoids from Clausena indica(Datz).Oliv showed good effect on removal of ·OH and DPPH·.This study provided application and development of Clausena indica(Datz).Oliv fruit. |
| Keywords: | Clausena indica(Datz) Oliv fruit total flavonoids ultrasonic extraction antioxidative activity |
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