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| 辽东楤木叶片皂苷超声提取工艺优化研究 | |
| 引用本文： | 黄福山,赵恒田,李富恒,邓中枢,王晓宇. 辽东楤木叶片皂苷超声提取工艺优化研究[J]. 食品工业科技, 2012, 33(6): 360-362,400 |
| 作者姓名： | 黄福山 赵恒田 李富恒 邓中枢 王晓宇 |
| 作者单位： | 1. 东北农业大学生命科学学院,黑龙江哈尔滨150030／中国科学院东北地理与农业生态研究所黑土区农业生态重点实验室,黑龙江哈尔滨150081 2. 中国科学院东北地理与农业生态研究所黑土区农业生态重点实验室,黑龙江哈尔滨,150081 3. 东北农业大学生命科学学院,黑龙江哈尔滨,150030 |
| 基金项目： | 中科院特殊经费支持项目(ZD08-1);黑龙江省科技成果推广项目(TC10B1003) |
| 摘 要： | 以辽东楤木叶片为试材,采用超声波提取法,以乙醇为提取溶剂提取并测定楤木皂苷含量。在单因素实验的基础上进行正交实验,研究叶片中楤木皂苷的最佳提取工艺。结果表明,叶片楤木皂苷的最佳提取工艺为乙醇浓度70%、溶剂倍量40、提取温度70℃、提取时间75min。采用最佳的超声提取工艺测定叶片中楤木皂苷含量为92.77mg/g。经优化的超声提取工艺操作简单、提取时间短、提取效率高且结果稳定。 |
| 关 键 词： | 辽东楤木 楤木皂苷 含量测定 超声提取 |
Optimization of ultrasonic extraction of aralosides in leaf of Aralia elata(Miq. ) Seem |
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| Affiliation: | HUANG Fu - shan1,2,ZHAO Heng - tian2,LI Fu - heng1,*,DENG Zhong - shu2,WANG Xiao - yu1 (1. College of Life Sciences,Northeast Agricultural University,Harbin 150030,China;2. Key Laboratory of Mollisols Agroecology,Northeast Institute of Geography and Agroecology,Chinese Academy of Sciences,Harbin 150081,China) |
| Abstract: | Aralosides were extracted by using Aralia elata(Miq. )Seem leaves as testing materials,using ethanol as solvent and determination content of aralosides by ultrasonic extraction. Based on the single - factor test,the optimal extraction conditions of aralosides in Aralia elata(Miq. )Seem leaves were studied by the orthogonal experiment. The results showed that the optimal extraction conditions were 70% ethanol concentration,40 multiples of solvent,an extraction temperature of 70℃,an extraction time of 75min. The extraction content of aralosides reached 92. 77mg/g under the optimal extraction conditions. The optimized ultrasonic extraction process was simple,rapid,efficient,and the result was stable. |
| Keywords: | Aralia elata(Miq. )Seem. araloside determination ultrasonic extraction |
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