| 蚕蛹蛋白中性蛋白酶可控水解动力学模型 |
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| 引用本文： | 赵钟兴,廖丹葵,孙建华,黄科林,孙果宋,秦伟佳,吕汶骏,李玲,吴志洪,童张法. 蚕蛹蛋白中性蛋白酶可控水解动力学模型[J]. 食品工业科技, 2012, 33(11): 97-100,103 |
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| 作者姓名： | 赵钟兴 廖丹葵 孙建华 黄科林 孙果宋 秦伟佳 吕汶骏 李玲 吴志洪 童张法 |
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| 作者单位： | 1. 广西大学化学化工学院,广西石油资源加工与过程强化重点实验室,广西南宁530004
2. 广西化工研究院,广西南宁,530004 |
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| 基金项目： | 广西自然科学基金项目(2010GXNSFA013058);广西研究生教育创新计划资助项目(105930901005);广西大学科研基金资助项目(XGL090021) |
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| 摘 要： | 提出了同时考虑产物抑制、底物抑制、酶失活等因素的机理模型,并应用到蚕蛹蛋白中性蛋白酶水解体系中,证明了在该体系中底物抑制现象的存在,并推导出相关动力学和热力学常数分别为Km=4.02g.L-1,k2=130.9min-1,Ks=110.4g.L-1,kd=24.6min-1,C0=1.38×10-4,rmax=15.4g.L-1.min-1和Ea=44.5kJ.mol-1,并通过实验结果比较验证模型的可靠性,结果表明计算值与实验值较为吻合,相对误差只有3.80%,说明该模型具有较好的推算性。
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| 关 键 词： | 动力学 蚕蛹蛋白 水解 酶 |
Kinetic model of controlled hydrolysis of proteins from silkworm pupae with neutrase |
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| ZHAO Zhong-xing,LIAO Dan-kui,SUN Jian-hua,HUANG Ke-lin,SUN Guo-song,QIN Wei-jia,LV Wen-jun,LI Ling,WU Zhi-hong,TONG Zhang-fa. Kinetic model of controlled hydrolysis of proteins from silkworm pupae with neutrase[J]. Science and Technology of Food Industry, 2012, 33(11): 97-100,103 |
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| Authors: | ZHAO Zhong-xing LIAO Dan-kui SUN Jian-hua HUANG Ke-lin SUN Guo-song QIN Wei-jia LV Wen-jun LI Ling WU Zhi-hong TONG Zhang-fa |
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| Affiliation: | 1,*(1.School of Chemistry and Chemical Engineering,Guangxi Key Laboratory of Petrochemical Resources Processing & Process Intensification Technology,Guangxi University,Nanning 530004,China; 2.Guangxi Research Institute of Chemical Industry,Nanning 530004,China) |
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| Abstract: | Taking into account the reaction mechanism including substrate inhibition,product inhibition,irreversible enzyme inactivation and critical enzyme concentration,a kinetic model was established to characterize the enzymatic hydrolysis curves.It proved that the substrate inhibition existed in this system,and its reaction and thermodynamic constants were determined as Km=4.02g·L-1,k2=130.9min-1,Ks=110.4g·L-1,kd=24.6min-1,C0=1.38×10-4,rmax=15.4g·L-1·min-1,Ea=44.5kJ·mol-1.At last,the experimental data were substituted this kinetic model,the regressive results agree well with the experimental data,i.e.the average relative error was only 3.80%.So,it proved that kinetic model was reasonable,and the whole set of exponential kinetic equations could be used to model the hydrolysis process of PSP-Neutrase. |
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| Keywords: | kinetic protein of silkworm pupae hydrolysis enzyme |
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