| 高速逆流色谱法分离制备孜然黄酮 |
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| 引用本文： | 吴素玲,孙晓明,张卫明,杨艳,张锋伦.高速逆流色谱法分离制备孜然黄酮[J].食品工业科技,2012,33(11):215-217. |
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| 作者姓名： | 吴素玲 孙晓明 张卫明 杨艳 张锋伦 |
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| 作者单位： | 南京野生植物综合利用研究院,江苏南京,210042 |
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| 基金项目： | 国家十一五科技支撑计划(2006BAD06B02) |
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| 摘 要： | 应用高速逆流色谱法分离制备了孜然中的黄酮类成分。以氯仿∶甲醇∶水=4∶4∶2(v∶v∶v)为两相溶剂系统,在主机转速为850r/min、流速1.8mL/min、检测波长254nm条件下进行分离制备。所得分离收集液经高效液相色谱法检测,结果表明,从孜然黄酮粗提物中分离得到了纯度超过90%的两种黄酮类成分;经干燥得样品重分别为26mg和24mg。
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| 关 键 词： | 高速逆流色谱 孜然 黄酮类成分 |
Separation and purification of the flavonoid from Cuminum cyminum L by high speed countercurrent chromatography |
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| WU Su-ling,SUN Xiao-ming,ZHANG Wei-ming,YANG Yan,ZHANG Feng-lun.Separation and purification of the flavonoid from Cuminum cyminum L by high speed countercurrent chromatography[J].Science and Technology of Food Industry,2012,33(11):215-217. |
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| Authors: | WU Su-ling SUN Xiao-ming ZHANG Wei-ming YANG Yan ZHANG Feng-lun |
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| Affiliation: | (Nanjing Research Institute for the Comprehensive Utilization of Wild Plants,Nanjing 210042,China) |
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| Abstract: | High-speed countercurrent chromatography(HSCCC) with a two-phase solvent system composed of chloroform-methanol-water(4∶4∶2) was applied to separate and purify two flavonoids from Cuminum cyminum L.The mobile phase was performed at a flow rate of 1.8mL/min,while the apparatus rotated at 850r/min and detected at 254nm.Two kinds of flavones were obtained and yielded 26mg and 24mg respectively.The results showed that the purities of two flavones were more than 90% determined by high performance liquid chromatography(HPLC) which was a simple and rapid method. |
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| Keywords: | high speed countercurrent chromatography(HSCCC) Cuminum cyminum L flavonoid |
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