油茶皂素的提取及纯化新方法

采用乙醇为浸提剂提取油茶籽饼粕中的油茶皂素,正交实验结果表明,油茶皂素提取的较优工艺条件为:乙醇浓度为50%,浸提温度60℃,料液比1:10,浸提时间2h,在此条件下油茶皂素的得率为16.66%,粗产品的油茶皂素的纯度为62.37%。用高速逆流色谱法对粗品进行分离纯化,初步的实验结果表明:乙酸乙酯:正丁醇:水(1:1:2,V/V/V)是适合于油茶皂素分离纯化的溶剂系统,能将油茶皂素含量提高到91.8%。

New study on extraction and purification of tea saponin

Tea saponin was extracted from seeds of camellia oleifera with alcohol as extraction solvent.The results of orthogonal test showed that the optimum technological conditions were as follows:ethanol concentration 50%,soaking temperature 60℃,ratio of liquid to solid 1:10 and soaking time 2h.Under the conditions,the tea saponin yield was 16.66%,and its purity in crude product was 62.37%.Then the crude tea saponin was further purificated and separated by HSCCC.The preliminary results showed that the solvent system of ethyl acetate-n-butanol-water(1:1:2,V/V/V)was suitable for the purification of tea saponin and the content can be increased to 91.8%.