| 不同因素对松花粉中脂肪酶活力的影响 |
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| 引用本文： | 刘瑞,谢霖霖,刘志河,王桐,石丽花,耿越.不同因素对松花粉中脂肪酶活力的影响[J].食品工业科技,2012,33(4):202-204. |
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| 作者姓名： | 刘瑞 谢霖霖 刘志河 王桐 石丽花 耿越 |
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| 作者单位： | 1. 山东师范大学生命科学学院,山东济南,250014
2. 烟台新时代健康产业（集团）有限公司,山东烟台,264006 |
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| 摘 要： | 为研究松花粉中脂肪酶的最适反应条件,考察了温度(℃)、pH、底物、金属离子等因素对其酶活的影响。通过脂肪酶的催化水解作用,以95%乙醇终止反应,测定其酸价,进而求得酶活,并选择出脂肪酶的最适反应条件。结果表明,最佳反应条件为:温度35℃,pH7.4,底物为三丁酸甘油酯,Ca2+、Fe3+、Mg2+、Fe2+会提高脂肪酶活力。
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| 关 键 词： | 松花粉 脂肪酶 影响因素 酶活力 |
Effect of different factors on lipase activity in Pine Pollen |
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| LIU Rui,XIE Lin-lin,LIU Zhi-he,WANG Tong,SHI Li-hua,GENG Yue.Effect of different factors on lipase activity in Pine Pollen[J].Science and Technology of Food Industry,2012,33(4):202-204. |
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| Authors: | LIU Rui XIE Lin-lin LIU Zhi-he WANG Tong SHI Li-hua GENG Yue |
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| Affiliation: | 1,*(1.College of Life Science,Shandong Normal University,Jinan 250014,China;2.Yantai New Era Healthy Industry(Group)Co.,Ltd.,Yantai 264006,China) |
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| Abstract: | In order to study the optimal conditions of lipase in Pine Pollen,we investigated some influence factors,including temperature(℃),pH,substrate,metal ions,and so on.The lipase activity was obtained by assaying of the acid value after ending the reaction with 95% ethanol and the optimal conditions were determined.The results showed that the optimum temperature and pH of lipase were 35℃ and 7.4 respectively,the substrate was glyceryl tributyrate,and Ca2+,Fe3+,Mg2+,Fe2+ enhanced the activity of lipase. |
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| Keywords: | Pine Pollen lipase influence factors enzymatic activity |
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