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谷氨酰胺转氨酶对碱溶酸沉法提取南极磷虾(Euphausia superba)蛋白质得率的影响
引用本文:王灵昭,薛长湖,王玉明,薛勇.谷氨酰胺转氨酶对碱溶酸沉法提取南极磷虾(Euphausia superba)蛋白质得率的影响[J].食品工业科技,2012,33(3):139-141.
作者姓名:王灵昭  薛长湖  王玉明  薛勇
作者单位:1. 中国海洋大学食品科学与工程学院,山东青岛266003 淮海工学院海洋学院,江苏连云港222005
2. 中国海洋大学食品科学与工程学院,山东青岛,266003
基金项目:国际科技合作项目(2010DFA31330);国家863计划项目(2011AA090801)
摘    要:通过碱溶酸沉法提取了南极磷虾蛋白质,研究了谷氨酰胺转氨酶的应用对蛋白质得率的影响。结果表明:碱溶酸沉法能够较好地回收南极磷虾蛋白质;在酸沉过程中,合理添加谷氨酰胺转氨酶能够提高蛋白质得率约5%。

关 键 词:南极磷虾  蛋白质得率  碱溶酸沉法  谷氨酰胺转氨酶

Effect of transglutaminase on yield of proteins extracted from whole Antarctic krill(Euphausia superba) by alkaline solubilization and acid precipitation method
WANG Ling-zhao,XUE Chang-hu,WANG Yu-ming,XUE Yong.Effect of transglutaminase on yield of proteins extracted from whole Antarctic krill(Euphausia superba) by alkaline solubilization and acid precipitation method[J].Science and Technology of Food Industry,2012,33(3):139-141.
Authors:WANG Ling-zhao  XUE Chang-hu  WANG Yu-ming  XUE Yong
Affiliation:1 (1.College of Food Science and Engineering,Ocean University of China,Qingdao 266003,China;2.School of Marine Science and Technology,Huaihai Institute of Technology,Lianyungang 222005,China)
Abstract:Alkaline solubilization and acid precipitation method was employed to extract proteins from whole Antarctic krill(Euphausia superba),and effect of transglutaminase on protein recovery yield was researched.Results indicated that the alkaline solubilization and acid precipitation method was good at recovering protein from whole Antarctic krill,and protein recovery yield was increased by approximate 5%,when transglutaminase was correctly applied during acid-induced protein precipitation.
Keywords:Antarctic krill  protein yield  alkaline solubilization and acid precipitation method  transglutaminase
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