| 分光光度法测定葡萄酒中尿素含量 |
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| 引用本文： | 梁新红,杨大光,孙俊良,郭祖峰,唐玉.分光光度法测定葡萄酒中尿素含量[J].食品工业科技,2012,33(4):76-79. |
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| 作者姓名： | 梁新红 杨大光 孙俊良 郭祖峰 唐玉 |
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| 作者单位： | 河南科技学院食品学院,河南新乡,453003 |
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| 基金项目： | 河南省教育厅自然科学研究资助计划项目(2010A550004);河南科技学院博士基金资助项目(08001) |
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| 摘 要： | 目的:建立了分光光度法定量检测葡萄酒中尿素含量的方法。方法:采用强阳性离子交换树脂柱对葡萄酒中尿素进行分离,依据费伦反应原理,采用分光光度法测定洗脱液中尿素含量。结果:建立方法的最佳参数为丁二酮单肟最佳添加量6.0g/L,氨基硫脲最佳添加量0.10g/L,反应中煮沸时间为5min,用分光光度计在530nm波长下测定吸光度。方法的最低检出限0.5mg/L,相对标准偏差为3.1%～4.4%,回收率为92.8%～103.1%。结论:此方法简便快速、准确可靠,适合用于葡萄酒样品中尿素含量的定量检测。
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| 关 键 词： | 分光光度法 葡萄酒 尿素 费伦试剂 |
Spectrophotometric determination of urea level in wine |
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| LIANG Xin-hong,YANG Da-guang,SUN Jun-liang,GUO Zu-feng,TANG Yu.Spectrophotometric determination of urea level in wine[J].Science and Technology of Food Industry,2012,33(4):76-79. |
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| Authors: | LIANG Xin-hong YANG Da-guang SUN Jun-liang GUO Zu-feng TANG Yu |
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| Affiliation: | (Food College,Henan Institute of Science and Technology,Xinxiang 453003,China) |
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| Abstract: | Objective:A method was described for colorimetric determination of urea in wine.Method:Urea in wine samples was extrated with the cation-exchange resin,and then urea in the elute was measured based on Fearon reaction with spectrophotometer.Result:The results showed that the optimal conditions as follows:the concentration of diacetyl monoxime 6.0g/L,thiosemicarbazide 0.10g/L,and the mixture boiled at 100℃ for 5min,and measure absorbance at 530nm.The determination limit of the method of wine samples was 0.5mg/L,and the RSD were 3.1% ～ 4.4%,and recoveries were 92.8% ～ 103.1%.Conclusion:The method presented in this paper was accurate and reliable,and it was suitable for determination of urea in wine. |
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| Keywords: | spectrophotometric method wine urea fearon reagents |
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