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林蛙皮胶原蛋白肽的制备工艺
引用本文:王长周,邱芳萍,房天琪,季晓峰.林蛙皮胶原蛋白肽的制备工艺[J].食品工业科技,2012,33(7):309-310,314.
作者姓名:王长周  邱芳萍  房天琪  季晓峰
作者单位:长春工业大学功能食品与生物技术研究所,吉林长春,130012
基金项目:吉林省中小企业创新基金项目(20106042)
摘    要:以长白山林蛙皮为原料,选用碱性蛋白酶,对酶解温度、酶解时间、加酶量、pH、料液比五个因素来研究,经实验确定最佳酶解条件为:酶解温度50℃,加酶量0.5%,酶解时间3h,pH9.0,料液比1∶3(g/mL),脱色脱腥处理后,喷雾干燥制得微黄色粉末状林蛙皮胶原蛋白肽。结果表明,羟脯氨酸含量为20.69μg/mL,分子量分布在3.5ku以下。

关 键 词:林蛙皮  胶原蛋白肽  制备  工艺

Preparation process for rana skin collagen peptide
WANG Chang-zhou,QIU Fang-ping,FANG Tian-qi,JI Xiao-feng.Preparation process for rana skin collagen peptide[J].Science and Technology of Food Industry,2012,33(7):309-310,314.
Authors:WANG Chang-zhou  QIU Fang-ping  FANG Tian-qi  JI Xiao-feng
Affiliation:(Research Laboratory of Functional Food and Biotechnology,Changchun University of Technology,Changchun 130012,China)
Abstract:The rana skin was used as a raw material.Alkali protease were chosen and the influences of the five process parameters which were enzyme solution temperature,enzymatic hydrolysis time,plus enzyme quantity,pH and liquid materials were studied.According to the experiment,the optimal media conditions were 50℃ temperature zymolysing,adding enzyme quantity 0.5%,zymolysing time 3h,pH9.0,1∶3 liquid to material rate(g/mL).After decoloring and deodorization processing,yellowish powder rana skin collagen peptide was got through spray drying.The results showed that hydroxyproline content was 20.69μg/mL and molecular weight was under 3.5ku.
Keywords:rana skin  collagen peptide  preparation  process
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