| 脂肪酶催化合成肉豆蔻酸植物甾醇酯 |
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| 引用本文： | 罗日明,曾朝喜,徐达,陈华勇,杨博. 脂肪酶催化合成肉豆蔻酸植物甾醇酯[J]. 食品工业科技, 2012, 33(9): 180-182 |
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| 作者姓名： | 罗日明 曾朝喜 徐达 陈华勇 杨博 |
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| 作者单位： | 华南理工大学生物科学与工程学院,广东广州,510006 |
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| 基金项目： | 广东省科技攻关(2009B020201007) |
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| 摘 要： | 考察了在有机介质中不同脂肪酶催化合成肉豆蔻酸植物甾醇酯,选定脂肪酶AYS为催化剂。单因素实验结果表明在正己烷体系中,最优的反应条件为肉豆蔻酸和植物甾醇摩尔比为3∶1,脂肪酶AYS用量为底物总质量的10%,反应温度50℃,反应72h后酯化率可达69.93%。
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| 关 键 词： | 植物甾醇 肉豆蔻酸 酶催化 植物甾醇酯 |
Lipase-catalyzed synthesis of phytosterol myristate |
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| LUO Ri-ming,ZENG Chao-xi,XU Da,CHEN Hua-yong,YANG Bo. Lipase-catalyzed synthesis of phytosterol myristate[J]. Science and Technology of Food Industry, 2012, 33(9): 180-182 |
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| Authors: | LUO Ri-ming ZENG Chao-xi XU Da CHEN Hua-yong YANG Bo |
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| Affiliation: | *(School of Bioscience and Bioengineering,South China University of Technology,Guangzhou 510006,China) |
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| Abstract: | Phytosterol myristate were synthesis from phytosterol and myristic acid by using various lipases as biocatalysts in organic media.The lipase AYS was selected for further experiments.Through the single factor experiment,the results showed that the yield of esterification could reach 69.93% in hexane.The optimal reaction conditions were as follows:molar ratio of myristic acid to phytosterol 3:1,lipase AYS load 10%(on the basis of the substrate mass),reaction temperature 50℃,reaction time 72h. |
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| Keywords: | phytosterol myristic acid lipase-catalyzed phytosterol myristate |
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