| 超声辅助酶法提取废次烟叶中绿原酸、烟碱工艺研究 |
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| 引用本文： | 朱松,娄在祥,陈尚卫,戴军,王洪新.超声辅助酶法提取废次烟叶中绿原酸、烟碱工艺研究[J].食品工业科技,2012,33(5):181-184. |
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| 作者姓名： | 朱松 娄在祥 陈尚卫 戴军 王洪新 |
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| 作者单位： | 1. 江南大学食品科学与技术国家重点实验室,江苏无锡,214122
2. 江南大学食品学院,江苏无锡,214122 |
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| 摘 要： | 将超声波技术与酶法相结合提取烟叶中的绿原酸和烟碱。通过实验得出最适宜的酶为纤维素酶;在单因素实验的基础上,通过正交实验优化超声波辅助酶法同时提取绿原酸和烟碱的最佳条件为:酶用量1%(以烟叶质量计)、酶解时间1.5h,酶解温度50℃。在最佳条件下,绿原酸的提取率为91.5%,烟碱的提取率为92.2%,与其他提取方法比较,超声波辅助酶法对烟叶中绿原酸、烟碱的提取具有明显的促进作用。
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| 关 键 词： | 烟叶 绿原酸 烟碱 超声波 酶 提取 |
Study on ultrasound-assisted enzyme extraction of chlorogenic acid and nicotin from tobacco leaves |
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| ZHU Song,LOU Zai-xiang,CHEN Shang-wei,DAI Jun,WANG Hong-xin.Study on ultrasound-assisted enzyme extraction of chlorogenic acid and nicotin from tobacco leaves[J].Science and Technology of Food Industry,2012,33(5):181-184. |
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| Authors: | ZHU Song LOU Zai-xiang CHEN Shang-wei DAI Jun WANG Hong-xin |
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| Affiliation: | 2,*(1.State Key Laboratory of Food Science and Technology,Jiangnan University,Wuxi 214122,China;2.School of Food Science & Technology,Jiangnan University,Wuxi 214122,China) |
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| Abstract: | Ultrasonic technique and enzyme were employed to extract chlorogenic acid and nicotin from tobacco leaves.The results indicated that ultrasonic technique and enzyme treatment significantly promoted the extraction of chlorogenic acid and nicotin.The optimum condition of extraction was obtained through orthogonal test.Experiment results showed that the optimum extraction parameters were:1.0% dry weight of amylase,extraction time of 1.5h,temperature of 50℃.Under this condition the extraction rate of chlorogenic acid was up to 91.5%,and nicotin was 92.2%. |
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| Keywords: | tobacco leaves chlorogenic acid nicotin ultrasonic technique enzyme extraction |
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