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苋菜多酚提取工艺及抗氧化研究
引用本文:周向军,高义霞,张永德,张继. 苋菜多酚提取工艺及抗氧化研究[J]. 食品工业科技, 2012, 33(6): 327-331
作者姓名:周向军  高义霞  张永德  张继
作者单位:1. 天水师范学院生命科学与化学学院,甘肃天水,741001
2. 天水师范学院生命科学与化学学院,甘肃天水741001/西北师范大学生命科学学院,甘肃兰州730070
基金项目:天水师范学院重点学科支持项目
摘    要:以苋菜为原料,利用正交实验研究了不同因素对苋菜多酚得率的影响,同时对苋菜多酚的抗氧化作用进行了探讨。结果表明,提取时间对苋菜多酚得率有显著影响,最佳提取工艺为:提取时间40min,提取温度40℃,固液比1∶25(g/mL),乙醇浓度50%,在此条件下重复三次,苋菜多酚得率为16.04%。抗氧化实验表明,苋菜多酚对羟自由基、超氧阴离子自由基、DPPH自由基均具有显著的清除作用(p<0.05),且清除作用随其浓度增大而增强。

关 键 词:苋菜  多酚  提取  抗氧化

Study on extraction technology and antioxidant activity of polyphenol from Amaranthus mangostanus L.
ZHOU Xiang - jun,GAO Yi - xia,ZHANG Yong - de,ZHANG Ji. Study on extraction technology and antioxidant activity of polyphenol from Amaranthus mangostanus L.[J]. Science and Technology of Food Industry, 2012, 33(6): 327-331
Authors:ZHOU Xiang - jun  GAO Yi - xia  ZHANG Yong - de  ZHANG Ji
Affiliation:1,2,* (1. College of Life Science and Chemistry,Tianshui Normal University,Tianshui 741001,China; 2. Department of Life Science,Northwest Normal University,Lanzhou 730070,China)
Abstract:Effect of different factors on polyphenol from Amaranthus mangostanus L. and its antioxidant activity were investigated. The results showed that extraction time had significant effect on polyphenol yield,and the optimum extraction conditions were obtained by orthogonal tests as follow:extraction time 40min,extraction temperature 40℃,solid - liquid ratio 1∶ 25(g/mL),ethanol concentration 50%. Under these conditions,polyphenol yield was 16. 04%. Antioxidant activity showed that polyphenol from Amaranthus mangostanus L. had significant scavenging action on hydroxyl radical,superoxide anion radical,DPPH free radical(p<0. 05),and its scavenging action increased with increasing concentration.
Keywords:Amaranthus mangostanus L.  polyphenol  extraction  antioxidant activity
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