| 高效液相色谱法测定西兰花中萝卜硫素的研究 |
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| 引用本文： | 顾颖娟,张亮,邹宇,陈志刚,韩永斌,顾振新. 高效液相色谱法测定西兰花中萝卜硫素的研究[J]. 食品工业科技, 2012, 33(4): 80-82,85 |
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| 作者姓名： | 顾颖娟 张亮 邹宇 陈志刚 韩永斌 顾振新 |
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| 作者单位： | 南京农业大学食品科技学院,江苏南京,210095 |
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| 基金项目： | 江苏省科技支撑计划项目(BE2006309) |
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| 摘 要： | 建立了采用高效液相色谱法(HPLC)测定西兰花种子及芽苗中萝卜硫素的方法。具体方法为:采用反相XDB-C18色谱柱分离,乙腈-水梯度(10%-60%-100%,0-25-30min)洗脱,流速0.6mL/min,检测波长254nm,进样量50μL。萝卜硫素测定结果的相对标准偏差小于1%,平均回收率达到97.14%。该方法具有较好的分离效果和较高的稳定性,适用于西兰花中萝卜硫素含量的测定。
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| 关 键 词： | 西兰花 高效液相色谱法 萝卜硫素 测定 |
Study on determination method of sulforaphane in broccoli by HPLC |
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| GU Ying-juan,ZHANG Liang,ZOU Yu,CHEN Zhi-gang,HAN Yong-bin,GU Zhen-xin. Study on determination method of sulforaphane in broccoli by HPLC[J]. Science and Technology of Food Industry, 2012, 33(4): 80-82,85 |
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| Authors: | GU Ying-juan ZHANG Liang ZOU Yu CHEN Zhi-gang HAN Yong-bin GU Zhen-xin |
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| Affiliation: | *(College of Food Science and Technology,Nanjing Agricultural University,Nanjing 210095,China) |
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| Abstract: | A high performance liquid chromatograph(HPLC) method had been set up for determination of content of sulforaphane in broccoli seeds and buds.The chromatographic separation was achieved on reversed XDB-C18 using acetonitrile-water as the mobile phase(10%-60%-100%,0-25-30min) at a flow rate of 0.6mL/min.The detector wavelength was set at 254nm and the injection volume used was 50μL.The relative standard deviation of sulforaphane content determinated was less than 1% and the average recovery was 97.14%.This method showed good separation effect and high stability,and could be used for determining the content of sulforaphane in broccoli. |
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| Keywords: | broccoli HPLC sulforaphane determination |
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