葡聚糖酶应用于甘蔗混合汁的工艺优化

制糖过程葡聚糖的存在不仅造成蔗糖损失,其高粘性也给生产带来严重的影响。利用响应面法(RSM)对葡聚糖酶降解糖厂混合汁中的葡聚糖进行了工艺优化。基于单因素实验,以葡聚糖酶剂量(mg/kg)、酶解pH、酶解温度(℃)、酶解时间(min)为响应因子,混合汁中葡聚糖含量为响应值,作四因素三水平响应面分析,得到酶解的最优工艺条件。结合糖厂实际生产,提出可供糖厂应用葡聚糖酶降解葡聚糖的参考工艺条件:葡聚糖酶剂量10～15mg/kg蔗汁,反应时间10～15min,反应pH为4.5～5.5(最优为4.90),反应温度为50～60℃(最优为54.3℃)。在此工艺范围内,得到混合汁中的葡聚糖含量为313～354mg/kg·Brix,对应其去除率为74.45%～71.10%。

Optimize the application of dextranase in sugarcane mixture

Not only does the formation of dextran brought in expensive sucrose losses,but also the high-viscosity associated with this polysaccharide often interrupted normal processing operations.The optimization of dextranase to hydrolyze dextran in sugarcane mixture was carried out using response surface methodology(RSM).A Box-Behnken factorial design with three factors and three levels,including five replicates at the centre point,was used to determine the influence of enzyme dosage(mg/kg),pH and temperature(℃) reaction time(min) on.Considering practice manufacturing process in sugar mills,the optimum operational conditions were as follows:enzyme dosage 10 ～ 15mg/kg,reaction time 10 ～ 15min,pH value 4.50 ～ 5.50(the optimum 4.90),and temperature 50.0～60.0℃(the optimum 54.30℃).In this condition,it got the result:the dextran content in mixture was 313 ～ 354mg/kg·Brix,the corresponding removal of dextran was 74.45% ～ 71.10%.