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| 乌贼墨抑制海洋鱼体腐败菌活性成分的分离 | |
| 引用本文： | 师莉莎,刘华忠,钟杰平,谢主兰,乐小炎,黄志成,潘江球.乌贼墨抑制海洋鱼体腐败菌活性成分的分离[J].食品工业科技,2012,33(11):120-123. |
| 作者姓名： | 师莉莎 刘华忠 钟杰平 谢主兰 乐小炎 黄志成 潘江球 |
| 作者单位： | 1. 广东海洋大学食品科技学院,广东湛江,524088 2. 广东海洋大学现代生化中心,广东湛江,524088 3. 广东海洋大学理学院,广东湛江,524088 |
| 基金项目： | 广东省科技计划项目(2010A020507001-85);广东海洋大学人才引进项目 |
| 摘 要： | 对乌贼墨中抑制海洋鱼体腐败菌活性成分进行了分离,并初步探讨了抑菌成分的理化特性。结果表明:乌贼墨粗提物经DEAE-纤维素和Sepharose 4B分离后获得具有较高活性的抑菌活性物质,分子量约为83589u,对四种海洋鱼体腐败菌（气单胞菌、黄杆菌、芽孢杆菌、肠杆菌）的平均抑菌率为62.36%。经紫外扫描（UV）和红外光谱（IR）分析,抑菌物质的主要成分为多糖。 |
| 关 键 词： | 乌贼墨 抑菌活性 相对分子量 光谱性质 |
Isolation of the inhibitory compounds from cuttlefish ink against putrefying bacteria derived from marine fish |
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| SHI Li-sha,LIU Hua-zhong,ZHONG Jie-ping,XIE Zhu-lan,LE Xiao-yan,HUANG Zhi-cheng,PAN Jiang-qiu.Isolation of the inhibitory compounds from cuttlefish ink against putrefying bacteria derived from marine fish[J].Science and Technology of Food Industry,2012,33(11):120-123. | |
| Authors: | SHI Li-sha LIU Hua-zhong ZHONG Jie-ping XIE Zhu-lan LE Xiao-yan HUANG Zhi-cheng PAN Jiang-qiu |
| Affiliation: | 1,*(1.College of Food Science & Technology,Guangdong Ocean University,Zhanjiang 524088,China; 2.Modern Biochemistry Centre,Guangdong Ocean University,Zhanjiang 524088,China; 3.College of Science,Guangdong Ocean University,Zhanjiang 524088,China) |
| Abstract: | The inhibitory compounds from cuttlefish ink against putrefying bacteria derived from marine fish were isolated to explore these physical and chemical properties.Results revealed that the inhibitory active component was harvested from crude extract from squid ink by DEAE-cellulose ion-exchange and sepharose 4B chromatography,and relative molecular weight was determined to be 83589u by gel filtration chromatograghy.The natural materials inhibited four putrefying bacterium including Aeromonas,Flavobacterium,Bacillus,Enterobacter,and the mean inhibition ratio was 62.36%.Moreover,the inhibitory material against putrefying bacterium was mainly appraised to be polysaccharide by UV and IR spectrum scanning. |
| Keywords: | squid ink antibacterial activity relative molecular weight spectrum |
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