响应面法优化超声波辅助提取芒果核中原花青素的研究

以芒果核为原料,采用响应面法优化芒果核中原花青素超声波辅助提取条件。通过单因素实验和BoxBehnken Design实验研究乙醇体积分数、料液比、提取时间、超声波功率四个变量对芒果核中原花青素响应值的影响程度。用响应面法得出四个考察因素最优工艺参数,即:乙醇体积分数68.8877%、料液比1:18.179、提取时间21.75min、超声波功率329.007W。采用最佳工艺参数提取芒果核中原花青素,原花青素平均得率为6.42%,与响应面法优化原花青素得率预测值6.51512%接近。为芒果核中原花青素和食品色素有效开发利用提供一定的借鉴作用。

Optimization of ultrasonic-assisted extraction of proanthocyanidins from mango seeds using response surface methodology

The study to optimize the ultrasonic-assisted extraction of proanthocyanidins from mango seeds and effects of ethanol concentration,ratio of raw material to solvent,extraction time and ultrasonic power was investigated respectively in single-factor tests on proanthocyanidins yield.The extraction conditions were optimized by the maximization of the yield through response surface methodology with four factors and three levels based on the principle of Box-Behnken design.The results showed that the optimal conditions were as follows:ethanol concentration 68.8877%,ratio of raw material to solvent 1:18.179,extraction time 21.75min and ultrasonic power 329.007W.Under the above conditions,the confirmed tests indicated that the extraction rate of proanthocyanidins was 6.42%,which was close relation with the predicted yield of the extraction rate of proanthocyanidins was 6.51512%.The study would provide reference for the effective utilization of proanthocyanidins and food colors from mango seeds.