乳酸菌在不同食品包装材料生物膜形成特性

目的:研究乳酸菌在不同材料成膜情况。方法:用液体培养基在同一温度、时间,分别加入复合塑料包装材料(HDPE)、纸盒包装内层的复合铝膜、复合金属铝包装材料、玻璃4种不同包装材料上进行培养,6d后分别用分光光度计法和扫描电镜法检测成膜情况。结果:实验菌株乳酸菌有相对较弱的成膜能力:用分光光度法测得复合塑料包装材料(HDPE)上的菌膜吸光度相对较大,玻璃次之,复合金属铝包装材料数值最小;通过扫描电镜观察得出相似结果。结论:乳酸菌在复合塑料包装材料上成膜最好;在金属铝上成膜最差。

Spectrophotometer and scanning electron microscopy study of lactic acid bacteria biofilm forming on different materials

Objective:To study the lactic acid bacteria forming on different materials. Methods:Plastics Packaging Materials(HDPE),aluminum composite inner carton packaging,composite metal materials and glass were added into liquid mediums respectively. Strains grow at the same temperature and time for 6d. Spectrophotometer and scanning electron microscopy were used for testing the biofilm formation. Results:Lactic acid bacteria had weak biofilm formation ability. Strains showed the best biofilm formation ability on composite plastic packaging materials(HDPE)than on the other materials. The second level biofilm formation ability was on the glass,and the most difficult biofilm formation was on the aluminum composite metal. Similar result could be observed by scanning electron microscopy. Conclusion:The lactic acid bacteria biofilm could form easier on the plastic packaging material than on the other materials,the most difficult bacteria biofilm formation was on metal.