表面增强拉曼光谱检测食源性细菌的增强方式

因食源性微生物污染引起的疾病已经成为一个重要的卫生问题。传统的检测方法存在着检测时间长、成本高等不足,而拉曼光谱作为一种生物指纹识别技术,在细菌快速鉴定方面具有很大潜力。但是,拉曼光谱信号十分微弱,给实际应用带来了困难。表面增强拉曼光谱(SERS)是将待测物与粗糙金属表面接近,使拉曼信号大大得到增强,使之在临床诊断和快速鉴定方面的应用成为可能。本文以表面增强拉曼光谱检测致病菌为出发点,对增强底物与致病菌的结合方式和结合位点进行了总结。主要的增强方式分2类,一类是分别在细胞外和细胞内实现SERS,另一类是将纳米材料功能化,在分子水平实现SERS。这些增强方式将有利于新的增强底物的开发和增强机理的研究。

The enhanced mode in SERS detection of food-borne bacteria

Food-borne illness caused by microbial contamination has become an important health issue.The traditional detection methods need more time and higher costs.Raman spectroscopy as a biometric fingerprint recognition technology has great potential in the rapid identification of bacteria.However,Raman signal was very weak and difficult for practical use.Surface-enhanced Raman(SERS) let analyte close to the rough metal surface,enhanced the Raman signal greatly,so that it would be used in the clinical diagnosis and rapid identification.In this paper,the enhanced mode of SERS detection of bacteria and the binding sites of pathogen to the enhancement reagent were summarized in the application of surface-enhanced Raman spectroscopy on detection pathogenic bacteria.There were two major enhancement patterns.One was achieving SERS in the extracellular and intracellular respectively;the other was achieving SERS at the molecular level by functionalizing the nano-materials,which would help develop the new enhanced substrate and learn more about the mechanism of SERS.