不同提取方法对蛹虫草多糖抗氧化性的影响

目的:明确沸水浴、超声波和微波提取方法对蛹虫草多糖提取得率和抗氧化性的影响。方法:用3种不同方法提取蛹虫草多糖,经醇沉、脱色、脱蛋白制成精多糖,用苯酚-硫酸法测定多糖含量,用清除羟基自由基能力测定抗氧化性,并对测定结果进行比较。结果:当多糖浓度为1.0mg/mL时,沸水浴和微波提取多糖的抗氧化性分别为95.36%和97.11%,超声波较差,为74.46%。结论:利用微波提取的蛹虫草多糖具有很好的抗氧化性,而且提取时间短,提取得率较高。

Empirical study of different extraction methods influence on the antioxidation of polysaccharide in cordyceps militaris

Objective: To clear the Cordyceps polysaccharide extraction yield and the impact of antioxidation with the three methods, boiling water bath, microwave and ultrasonic extraction methods. Methods: With three different methods, we extract the polysaccharide. extracted by alcohol precipitation, decolorizing, removed protein to make the fine polysaccharide. phenol-sulphate acid to get the polysaccharide content, judged by the ability of removing the hydroxyl radical to measure the antioxidation, then compared with the two results. Results: When the concentration of polysaccharide is 1.0 mg/mL, the impact of polysaccharide antioxidation with boiling water bath and microwave extraction were 95.36% and 97.11%, the result of ultrasonic extraction is worst, just 74.46 percent. Conclusion: The polysaccharide extracted by microwave has the better antioxidation, and the process we would be with a shorter time, and have a higher rate.