| 液相色谱法测定谷物中脱氧雪腐镰刀菌烯醇 |
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| 引用本文： | 刘旭,李尧,龚珊,任正东,赵鹏,陈晓峰. 液相色谱法测定谷物中脱氧雪腐镰刀菌烯醇[J]. 西部粮油科技, 2010, 0(6): 92-94 |
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| 作者姓名： | 刘旭 李尧 龚珊 任正东 赵鹏 陈晓峰 |
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| 作者单位： | 陕西省粮油产品质量监督检验所,西安710016 |
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| 摘 要： | 建立检测谷物中脱氧雪腐镰刀菌烯醇(DON)的液相色谱方法,通过不同提取方法的比对来确定最佳的DON提取方案,并最终确定使用乙腈-水(体积比V/V,84/16)提取,经过DON专用净化柱的净化处理后,采用紫外检测器(λ=220 nm)及C18亲水分离柱测定。结果表明,用高速均质机均质4 min提取,可大幅度提高方法回收率,回收率达到90.7%~98.9%,方法最低检出限为50μg/kg。
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| 关 键 词： | 脱氧雪腐镰刀菌烯醇（DON） 液相色谱法 谷物 |
Determination of Deoxynivalenol in Grains by HPLC |
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| LIU Xu,LI Yao,GONG Shan,REN Zheng-dong,ZHAO Peng,CHEN Xiao-feng. Determination of Deoxynivalenol in Grains by HPLC[J]. China Western Cereals & Oils Technology, 2010, 0(6): 92-94 |
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| Authors: | LIU Xu LI Yao GONG Shan REN Zheng-dong ZHAO Peng CHEN Xiao-feng |
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| Affiliation: | (Shaanxi Province Grain ＆ Oil Products Quality Supervise Test Institute, Xi＇an 710016 ) |
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| Abstract: | A HPLC method was developed for deoxynivalenol （DON） in grains. Extraction was carried out with aceronitrile-water mixture （84/16,V/V）, followed by a clean-up step with TRILOGY columns. The extracts was analyzed by UV detector （λ = 220 nm） and C18 column. The results showed that using high-speed Homogenizer extracts 4 min, greatly increased the recovery rate（90.3%-102.1%）,the lowest detection limits was 50 μg/kg. |
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| Keywords: | deoxynivalenol HPLC grains |
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