| 产低温碱性脂肪酶菌株Acinetobacter johnsonii LP28的鉴定及发酵条件优化 |
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| 引用本文： | 戚薇,邵静,王晨,王海宽. 产低温碱性脂肪酶菌株Acinetobacter johnsonii LP28的鉴定及发酵条件优化[J]. 天津轻工业学院学报, 2009, 0(6): 9-12 |
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| 作者姓名： | 戚薇 邵静 王晨 王海宽 |
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| 作者单位： | 工业微生物教育部重点实验室天津科技大学生物工程学院,天津300457 |
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| 基金项目： | 天津市自然科学基金资助项目（09JCZDJC17800） |
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| 摘 要： | 从实验室保藏的49株碱性脂肪酶产生菌中筛选出产低温碱性脂肪酶菌株LP28，初步酶学性质研究表明，该脂肪酶的最适作用温度为30℃，最适作用pH为9．0，粗酶液在室温条件下处理48h仍具有93．78％的残余酶活．该菌经16SrDNA鉴定为约氏不动杆菌（Acinetobacter johnsonii），同源性为99％．摇瓶实验表明，该菌株最适产酶培养基为（g/L）：淀粉10，牛肉膏20，K2HPO41，PvA-大豆油20．最高酶活为3．06U／mL．
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| 关 键 词： | 约氏不动杆菌 低温碱性脂肪酶 优化 |
Identification and Optimization of Fermentation Conditions of Acinetobacterjohnsonii LP28 Producing Cold-Adapted and Alkaline Lipase |
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| QI Wei,SHAO Jing,WANG Chen,WANG Hai-kuan. Identification and Optimization of Fermentation Conditions of Acinetobacterjohnsonii LP28 Producing Cold-Adapted and Alkaline Lipase[J]. Journal of Tianjin University of Light Industry, 2009, 0(6): 9-12 |
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| Authors: | QI Wei SHAO Jing WANG Chen WANG Hai-kuan |
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| Affiliation: | (Key Laboratory of Industrial Microbiology,Ministry of Education, College of Biotechnology, Tianjin University of Science ＆ Technology,Tianjin 300457 ,China) |
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| Abstract: | Strain LP28 was selected from the 49 alkaline lipase producing strains preserved in laboratory. The characterization of the crude enzyme exhibited maximum activity at 30 ℃ and pH 9.0 and retained 93.78% of its maximum activity after remaining 48 h at the room temperature. The strain was identified as Acinetobacter johnsonii by 16S rDNA assay and showed 99% homology. The optimal medium for lipase production was determined as follow （g/L） ：starch 10,beef extract 20 ,K2HPO4 1 ,PVA-bean oil 20. The alkaline lipase activity reached maximal level of 3.06 U/mL. |
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| Keywords: | Acinetobacterjohnsonii cold-adapted and alkaline lipase optimization |
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