| 低温碱性脂肪酶产生菌的筛选及产酶培养基的优化 |
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| 引用本文： | 刘瑞娟,王海宽,路福平,戚薇.低温碱性脂肪酶产生菌的筛选及产酶培养基的优化[J].天津轻工业学院学报,2009(1):6-10. |
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| 作者姓名： | 刘瑞娟 王海宽 路福平 戚薇 |
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| 作者单位： | 工业微生物教育部重点实验室天津科技大学生物工程学院,天津300457 |
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| 基金项目： | 天津市应用基础研究计划面上项目资助（07JCYBJC07900） |
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| 摘 要： | 以橄榄油为唯一碳源，从渤海湾盐碱地被油污染的土样中分离筛选出1株产低温碱性脂肪酶菌株34-5，初步酶学性质研究表明，该菌所产脂肪酶的最适温度为25℃，最适pH为9．6，该菌的16S rDNA基因序列与伯克霍尔德氏菌（Burkholderia）的同源性为99％摇瓶实验表明，该菌株最适产酶培养基为（g／L）；淀粉10，黄豆饼粉20，玉米浆20，K2HPO4 1，聚乙烯醇大豆油乳化液20．其最高酶活为6．87U／mL。
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| 关 键 词： | 低温脂肪酶 碱性脂肪酶 筛选 |
Screening and Optimization of Fermentation Medium of Bacterium Producing Cold-Adapted and Alkaline Lipase |
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| LIU Rui-juan,WANG Hai-kuan,LU Fu-ping,QI Wei.Screening and Optimization of Fermentation Medium of Bacterium Producing Cold-Adapted and Alkaline Lipase[J].Journal of Tianjin University of Light Industry,2009(1):6-10. |
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| Authors: | LIU Rui-juan WANG Hai-kuan LU Fu-ping QI Wei |
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| Affiliation: | (Key Laboratory of Industrial Microbiology, Ministry of Education, College of Biotechnology Tianjin University of Science ＆ Technology, Tianjin 300457, China) |
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| Abstract: | A bacterium which produces cold-adapted alkaline lipase was isolated from soil on a selective medium that contained olive oil as the only source of carbon. The soil was collected from bay of Bohai. The optimal temperature and pH of the lipase were 25℃ and pH 9.6, respectively. The sequence of 16S rDNA of strain 34-5 showed 99% homology to Burkholderia. The optimal medium for lipase production were determined as follows （g/L） ：starch 10, bean flour 20, corn syrup 20, K2HPO4 1, emulsioned bean oil 20. The alkaline lipase activity reached maximal level of 6.87 U/mL. |
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| Keywords: | cold-adapted lipase alkaline lipase screening |
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