铜绿微囊藻(Microcystic aeruginosa)胞内酸性多糖和胞外酸性多糖理化性质的比较研究

目的:研究比较铜绿微囊藻(Microcystic aeruginosa)胞内酸性多糖(IAPS)和胞外酸性多糖(EAPS)的理化性质、组分和结构,旨在将蓝藻水华作为一种天然植物资源进行综合开发利用。方法:采取不同的方法提取、分离、纯化得到IAPS和EAPS,并分别通过多种化学手段和HPLC、UV、IR、13CNMR等方法对IAPS和EAPS的分子量、单糖组成、糖醛酸、硫酸根等酸性基团和波谱学特征等进行了测定分析。结果硫酸咔唑法测定表明:IAPS糖醛酸含量为12.75%,而EAPS糖醛酸含量为10.74%;HPLC测定显示IAPS分子量(2.0025×105D)明显高于EAPS(7.0012×104D);IAPS主要由31.8341%D(+)木糖、33.0053%D-鼠李糖、4.1284%D-半乳糖、21.8689%L-阿拉伯糖,EAPS则由9.6549%D(+)木糖、19.2522%D-果糖、71.0930%葡萄糖;红外光谱和核磁共振图谱表明两者在结构上存在着显著的差异,这为研究两者的生理活性提供了物质基础。

Comparison of Physical and Chemical Characteristics between Intracellular and Extracellular Acidic Polysaccharides Extracted from Microcystic aeruginosa

Objective: Comparison of physical and chemical characteristics between intracellular and extracellular polysaccha- rides extracted from Microcystic aeruginosa was studied in order to be availably used as profitable resource. Methods: IAPS and EAPS were obtained by different methods of extraction, isolation and purification. HPLC, UV, IR, and 13CNMR etc. were used for the analyses of molecular weight, monosaccharide constituents and spectroscopic characteristics of IAPS and EAPS. Results: The mean molecular weight of IAPS (2.0025×105D) was heavier than that of EAPS (2.0025×105D). IAPS consisted of 12.75% uronic acid while EAPS I consisted of 10.74% uronic acid. The monosaccharide constituents of IAPS are D(+) xylose (31.8341%), D-rhamnose (33.0053%), D-galactose (4.1284%), L-arabinose (21.8689%) and those of EAPS are D(+) xylose (9.6549%), D-fructose (19.2522%), glucose (71.0930%). Analysis results of IR and 13C-NMR of these two IAPS and EAPS also demonstrate that their structures and monosaccharide constituents are obviously different. Conclusion: The physical character- istics of IAPS and EAPS are different.