| 吉林产贝母生物碱不同提取方法的HPLC指纹图谱分析 |
| |
| 引用本文： | 王世曼,于源华,蔡林君,王金凤,黎捷. 吉林产贝母生物碱不同提取方法的HPLC指纹图谱分析[J]. 长春理工大学学报(自然科学版), 2006, 29(3): 64-68 |
| |
| 作者姓名： | 王世曼 于源华 蔡林君 王金凤 黎捷 |
| |
| 作者单位： | 长春理工大学,生命科学技术学院,长春,130022;长春理工大学,计算机科学与技术学院,长春,130022 |
| |
| 摘 要： | 目的:探索吉林产贝母生物碱的最佳提取方法,为吉林产贝母生物碱成分的定性定量分析提供有效的方法。方法:利用高效液相色谱法(HPLC)对吉林产贝母的生物碱成分进行定性定量研究。结论:结果表明吉林产贝母药材的不同溶剂及不同方法提取液的HPLC图谱有显著差异。经氯仿:甲醇(4:1)混合溶剂索式提取的贝母药材HPLC图谱效果最好,其它次之。HPLC方法简便,快捷,结果可靠,灵敏度高,可用于贝母药材的质量控制。
|
| 关 键 词： | 吉林产贝母 生物碱 HPLC 指纹图谱 |
| 文章编号： | 1672-9870（2006）03-0064-05 |
| 收稿时间： | 2006-05-15 |
| 修稿时间： | 2006-05-15 |
Studys on HPLC Finger Print Chromatogram of Alkaloids of Fritillaria Jilin by different extraction method |
| |
| WANG Shiman,YU Yuanhua,CAI Linjun,WANG Jinfeng,LI Jie. Studys on HPLC Finger Print Chromatogram of Alkaloids of Fritillaria Jilin by different extraction method[J]. Journal of Changchun University of Science and Technology, 2006, 29(3): 64-68 |
| |
| Authors: | WANG Shiman YU Yuanhua CAI Linjun WANG Jinfeng LI Jie |
| |
| Abstract: | Aim: Search for the optimum extract method of Fritillaria Jilin in order to provide to effective method for qualitative analysis of alkaloids of Fritillaria Jilin. Methods:In this paper, the nature and quantity of Fritillaria Jilins component were studied by using of HPLC . Conclusion: The HPLC spectra of Fritillaria Jilin, extracted with different solvents and methods have notable difference. Furthermore, we found the best HPLC spectrum of Fritillaria Jilin was soxhlet method with the solvent of chloroform and methanol (4:1), the others were no better than it. The method of the HPLC has a lot of advantages, such as simple, shortcut, credible, sensitive and it can be used in controlling the Fritillaria Jilin's quality. |
| |
| Keywords: | Fritillaria Jilin alkaloids HPLC fingerprint chromatogram |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
