Laccase‐containing ureasil–polymer composite as the sensing layer of an amperometric biosensor |
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| Authors: | Taras Kavetskyy Oleh Smutok Mykhailo Gonchar Olha Demkiv Halyna Klepach Yuliia Kukhazh Ondrej ?au?a Tamara Petkova Victor Boev Vania Ilcheva Plamen Petkov Andrey L Stepanov |
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| Affiliation: | 1. Drohobych Ivan Franko State Pedagogical University, Drohobych, Ukraine;2. The John Paul II Catholic University of Lublin, Lublin, Poland;3. Institute of Cell Biology, National Academy of Sciences of Ukraine, Lviv, Ukraine;4. Institute of Physics, Slovak Academy of Sciences, Bratislava, Slovak Republic;5. Institute of Electrochemistry and Energy Systems, Bulgarian Academy of Sciences, Sofia, Bulgaria;6. Thin Films Technology Laboratory, University of Chemical Technology and Metallurgy, Sofia, Bulgaria;7. Kazan Physical‐Technical Institute, Russian Academy of Sciences, Kazan, Russian Federation |
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| Abstract: | Innovative amperometric biosensors for monitoring the level of wastewater pollution have been constructed on the surface of the gold planar electrodes C220AT “DropSens” by using the organic–inorganic ureasil‐based composites as host matrixes and immobilized commercial laccase from Trametes versicolor . It was found that the biosensor based on the ureasil–chalcogenide glass composite is characterized by a very high sensitivity (67,540 А M?1 m?2) that is 38.3 times higher than for pure ureasil (the sensitivity of the bioelectrode was calculated as 1762 А M?1 m?2). On the other hand, application of the ureasil–chalcogenide glass composite with incorporated silver nanoparticles (NPs) synthesized by high‐dose (1.0 × 1017 Ag+/cm2) 30 keV Ag+ ion implantation results in decreasing the biosensor sensitivity up to 2390 times (the sensitivity of the bioelectrode was 28.3 А M?1 m?2). The role of additives (chalcogenide glass and silver NPs) in the ureasil matrix on the biofunctionality of the biosensors produced is considered. © 2017 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2017 , 134 , 45278. |
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| Keywords: | amperometric biosensor laccase organic‐inorganic ureasil polymer silver nanoparticles ureasil‐chalcogenide glass composite |
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