| 光敏化BPD-MA后膀胱癌细胞凋亡的研究 |
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| 引用本文： | 许川山,吴士明,虞乐华,王志刚,周媛,杨青.光敏化BPD-MA后膀胱癌细胞凋亡的研究[J].激光技术,2006,30(1):78-81. |
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| 作者姓名： | 许川山 吴士明 虞乐华 王志刚 周媛 杨青 |
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| 作者单位： | 重庆医科大学,超声影像学研究所,重庆,400010;第三军医大学,新桥医院,重庆,400037;重庆医科大学,附属第二医院,康复中心,激光研究室,重庆,400010;第三军医大学,西南医院,整形外科中心,重庆,400038 |
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| 基金项目： | 中国科学院资助项目;军队科研项目 |
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| 摘 要： | 为了研究光动力抑癌作用机制,采用流式细胞仪和TUNEL法检测激光光敏化BPD-MA后人膀胱癌细胞株BIU-87细胞凋亡的发生状况.激光光敏化BPD-MA后人膀胱癌细胞株BIU-87组细胞凋亡发生率明显增高,许多细胞核呈棕黄色,高倍镜下可见细胞核内有大量棕褐色颗粒,与阳性对照中凋亡细胞核的特征相符合,而对照组此种细胞少见.TUNEL阴性对照中细胞核均呈蓝色.结果表明,激光激活BPD-MA光动力作用明显诱导人膀胱癌细胞株BIU-87细胞发生凋亡,这可能是其抑癌作用机制之一.
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| 关 键 词： | 医用光学与生物技术 BPD-MA 光动力作用 膀胱癌细胞 细胞凋亡 |
| 文章编号： | 1001-3806(2006)01-0078-04 |
| 收稿时间： | 2004-12-16 |
| 修稿时间： | 2005-01-25 |
Apoptosis of human bladder cancer BIU-87 cells after photodynamic therapy with laser-activated BPD-MA determined with TUNEL assays |
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| XU Chuan-shan,WU Shi-ming,YU Le-hua,WANG Zhi-gang,ZHOU Yuan,YANG Qing.Apoptosis of human bladder cancer BIU-87 cells after photodynamic therapy with laser-activated BPD-MA determined with TUNEL assays[J].Laser Technology,2006,30(1):78-81. |
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| Authors: | XU Chuan-shan WU Shi-ming YU Le-hua WANG Zhi-gang ZHOU Yuan YANG Qing |
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| Affiliation: | 1. Institute of Ultrasound Imaging, Chongqing University of Medical Sciences, Chongqing 400010, China; 2. Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China; 3. Department of Laser Research, Rehabilitation Center, Second Attiliated Hospital, Chongqing University of Medical Sciences, Chongqing 400010, China; 4. Center of Plastic Surgery, Southwest Hospital ,Third Military Medical University,Chongqing 400038, China |
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| Abstract: | In order to study the mechanism of photodynamic therapy on malignant tumors,photosensitization of BPD-MA is activated by laser with red light(632.8nm) delivered at 10mW/cm~2 to give a total dose of 2.4J/cm~2.Apoptosis of human bladder cancer BIU-87 cells is determined with flow cytometry with PI staining and terminal deoxyuridine nick end labeling(TUNEL) assays.Results show that photodynamic therapy with BPD-MA significantly increases the rate of apoptosis in BIU-87 cells.A lot of the positive cells in which their nucleus is brown-yellow fine particle shape or overflowing-dispersed are observed after treatment of cells with photodynamic therapy with BPD-MA.However,laser irradiation alone,BPD-MA alone and sham radiation without BPD-MA can not affect apoptosis of human bladder cancer BIU-87 cells.A conclusion can be made that treatment of cells with photodynamic therapy obviously induces human bladder cancer BIU-87 cells to apoptosis,which may be one of the important mechanism of photodynamic therapy on malignant tumors. |
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| Keywords: | BPD-MA |
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