| 血红蛋白分子印迹膜吸附性能的QCM评价 |
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| 引用本文： | 曾弢,方桂杰,刘立坚,谢卫红.血红蛋白分子印迹膜吸附性能的QCM评价[J].武汉理工大学学报,2011(5):63-67. |
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| 作者姓名： | 曾弢 方桂杰 刘立坚 谢卫红 |
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| 作者单位： | 湖北工业大学生物工程学院发酵工程教育部重点实验室; |
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| 基金项目： | 国家自然科学基金(20875024/B0509); 湖北省教育厅重大研究项目(Z20081402) |
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| 摘 要： | 以牛血红蛋白作为模板蛋白分子,在紫外光照射的条件下以偶氮二异丁腈(AIBN)作为光引发剂在石英晶体微天平(QCM)上分别制备了甲基丙烯酸甲酯(MMA)-三乙二醇二甲基丙烯酸酯(TEGDMA)、三乙二醇二甲基丙烯酸酯聚合膜,利用石英微天平表征了不同分子印迹膜对模板蛋白的响应信号,并对两种不同聚合膜材料表面对牛血红蛋白的吸附能力做了比较。结果表明,基于分子印迹技术构建的石英晶体微天平生物传感器具有良好的识别血红蛋白的特性。
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| 关 键 词： | 石英晶体微天平 分子印迹聚合膜 传感器 吸附能力 |
Evaluation of the Bovine Hemoglobin Molecularly Imprinted Polymer Membrane by QCM |
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| ZENG Tao,FANG Gui-jie,LIU Li-jian,XIE Wei-hong.Evaluation of the Bovine Hemoglobin Molecularly Imprinted Polymer Membrane by QCM[J].Journal of Wuhan University of Technology,2011(5):63-67. |
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| Authors: | ZENG Tao FANG Gui-jie LIU Li-jian XIE Wei-hong |
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| Affiliation: | ZENG Tao,FANG Gui-jie,LIU Li-jian,XIE Wei-hong(Key Laboratory of Fermentation Engineering for Ministry of Education of China,Institute of Bioengineering,Hubei University of Technology,Wuhan 430068,China) |
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| Abstract: | By utilizing bovine hemoglobin as template,AIBN as photo initiator,TEGDMA-MMA(TEGDMA as crosslinker and MMA as functional monomer) and TEGDMA molecularly imprinted polymer(MIP) menbranes were synthesized on the quartz crystal microbalance under the condition of UV-irradiation,respectively,response signal of different membranes to template protein was characterized by quartz crystal microbalance(QCM),and the capacity of two different MIP material membrane surfaces on the adsorption of bovine hemoglobin were ... |
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| Keywords: | quartz crystal microbalance molecularly imprinted polymer membrane biosensor adsorption capacity |
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