| 克雷伯氏菌属降解羟肟酸捕收剂H_(205)的研究 |
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| 引用本文： | 许念,龚文琪,梅光军,王灿.克雷伯氏菌属降解羟肟酸捕收剂H_(205)的研究[J].武汉理工大学学报,2011(8). |
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| 作者姓名： | 许念 龚文琪 梅光军 王灿 |
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| 作者单位： | 武汉理工大学资源与环境工程学院; |
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| 基金项目： | 国家“863”计划(2007AA06Z123); 污染物分析与资源化技术湖北省重点实验室开放基金 |
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| 摘 要： | 采用从汽车修理厂土壤中采集的菌种,借鉴国内外对多环芳烃化合物降解菌的驯化和筛选的方法,在自然条件下驯化、分离、筛选出能降解H205的菌株。研究各单因素对此优势降解菌的生物降解特性的影响,找出此优势降解菌的最佳降解条件。结果表明,从汽车修理厂土壤驯化而来的H205降解菌是革兰氏阴性菌,经16SrRNA鉴定为克雷伯氏菌属;该菌降解H205的最佳条件为:pH 6～7,温度30℃,摇床转速150r/min,细菌接种量10%;底物浓度100mg/L;外加碳源葡萄糖、蔗糖、乙酸钠都对H205的降解有抑制作用;外加氮源酵母膏、蛋白胨对H205的降解有促进作用。
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| 关 键 词： | 羟肟酸 H205 生物降解特性 |
Research on Biodegradation of Hydroxamic Acid Collector H_(205) by Klebsiellasp |
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| XU Nian,GONG Wen-qi,MEI Guang-jun,WANG Can.Research on Biodegradation of Hydroxamic Acid Collector H_(205) by Klebsiellasp[J].Journal of Wuhan University of Technology,2011(8). |
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| Authors: | XU Nian GONG Wen-qi MEI Guang-jun WANG Can |
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| Affiliation: | XU Nian,GONG Wen-qi,MEI Guang-jun,WANG Can(School of Resources and Environmental Engineering,Wuhan University of Technology,Wuhan 430070,China) |
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| Abstract: | Bacteria collected from soil in a vehicle repair plant were used with the method in acclimatization and screening of bacteria for PHAs degradation home and abroad.Under natural conditions,strains that can degrade hydroxamic acid collector H205 were cultivated through acclimation,isolation and screening.And this paper studied the effect on this dominant bacteria's biological degradation to induce the best degradation conditions.The results show that the bacterium domesticated from a vehicle repair plant soil... |
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| Keywords: | hydroxamic acids H205 biological degradation |
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