面包酵母冻干粉保护剂筛选及其生物活性分析

目的:优化真空冷冻干燥过程中保护剂配方,得到发酵性能较好的面包酵母3G-28冻干粉.方法:在对脱脂奶粉、蔗糖、吐温-80、阿拉伯胶、β-环状糊精、甘油单因素实验基础上,采用响应面实验对复合冻干保护剂的配方进行了优化,并对酵母冻干粉的发酵力、海藻糖含量、蔗糖酶活力以及发酵液中的风味物质进行了研究.结果:各因素对面包酵母冻...

Screening of Protective Agent and Bioactivity Analysis of Bread Yeast Freeze-dried Powder

Objective:The formulation of protective agent added in vacuum freeze-drying process was optimized to obtain bread yeast 3G-28 freeze-drying powder with good fermentation performance.Methods:On the basis of single factor experiments of skim milk powder,sucrose,Tween-80,Arabic gum,cyclodextrin and glycerin,the formula of compound freeze-drying protectant was optimized by response surface experiment,and the ferment power,trehalose content,sucrase activity and flavor in fermentation liquid of yeast freeze-drying powder were studied.Results:The order of influence of each factor on the number of viable bacteria in bread yeast freeze-dried powder was as follows:The concentration of glycerol>the concentration of sucrose>the concentration of skim milk powder>the concentration ofβ-cyclodextrin.The formula of the best compound freeze-drying protective agent was as follows:Glycerin 4.7%,skimmed milk powder 20%,β-cyclodextrin 15%,sucrose 5%.The viable quantity of bread yeast freeze-dried powder was 36.89×109 PCS/mL.The fermentation capacity of baker's yeast 3G-28 freeze-dried powder was 214 mg/h/g,the content of trehalose was 44.22 mg/g,and the activity of sucrase was 20.27 U/g.Compared with the freeze-dried powder of commercially available yeast and the freeze-dried powder of the initial strain of yeast,the yeast showed higher biological activity.In terms of flavor,the content of alcohol in bread yeast 3G-28 freeze-dried powder was the highest compared with the other two,showing good practicability.ConclusionThe optimum formulation of the baker's yeast 3G-28 freeze-drying powder was studied,and the baker's yeast freeze-drying powder with better biological activity was obtained,which had a good prospect in improving the quality of bread products and in the application of microbial industrialization.