双蛋白对大鼠血脂的影响研究

目的 探讨双蛋白对高脂模型大鼠血脂影响及作用机制。 方法 饲喂高脂饲料制备高脂模型,造模后根据血液生化指标分为空白对照组、高血脂模型组、双蛋白低剂量组(0.83g/kg)、双蛋白中剂量组(1.67g/kg)、双蛋白高剂量组(5.00g/kg)五组。连续灌胃6周后处死,测定大鼠血清中总胆固醇(total cholesterol,TC)、总甘油三酯(total glyceride,TG)、低密度脂蛋白胆固醇(low Density Lipoprotein,LDL)、高密度脂蛋白胆固醇(high density liptein cholesterol,HDL-C)。ELISA测定乙酰辅酶A羧化酶(acetyl-CoA carboxylase,ACC)、胰高血糖素(glucagon,GC)、胰岛素(insulin,INS)、3-羟基-3-甲酰基-CoA还原酶(HMG-CoA)含量。 结果 与空白组比较模型组除HMG-CoA外其他指标均有显著差异；与模型组相比,高剂量组LDL-C、TG、TC含量显著下降(P<0.05),GC、HDL-C、INS含量显著提升(P<0.05),各组HMG-CoA水平无显著差异。 结论 双蛋白可降低模型大鼠血脂水平,具有改善血脂异常的功能。其作用机制可能与调节GC、ACC等水平有关。

Study on the effect of double protein on blood lipid in rats

Objective To explore the effect and mechanism of double protein on blood lipid in hyperlipidemic model rats. Methods the high-fat model was made by feeding high-fat diet. After modeling, the model was divided into five groups according to blood biochemical indexes: blank control group, high-fat model group, double protein low-dose group (0.83g/kg), double protein medium dose group (1.67g/kg), double protein high-dose group (5.00g/kg). After continuous gavage for 6 weeks, the rats were killed and the serum levels of total cholesterol (TC), total triglyceride (TG), low density lipoprotein cholesterol (LDL) and high density lipoprotein cholesterol (HDL-C) were measured. The contents of acetyl CoA carboxylase (ACC), glucagon (GC), insulin (INS) and 3-hydroxy-3-formyl-coa reductase (HMG-CoA) were determined by ELISA. Results Compared with the blank group, the model group had significant differences in other indicators except HMG-CoA; Compared with the model group, the contents of LDL-C, TG and TC in the high-dose group decreased significantly (P<0.05), and the contents of GC, HDL-C and INS increased significantly (P<0.05). There was no significant difference in HMG-CoA levels among the groups. Conclusion the double protein can reduce the blood lipid level of model rats and has the function of improving dyslipidemia. The mechanism of action may be related to the regulation of GC and ACC levels.