白肉灵芝化学成分及体外抗氧化活性分析

为研究白肉灵芝的主要化学成分及抗氧化活性,本研究以紫芝、赤芝为参照,采用多种分析方法对白肉灵芝的营养成分、多糖、三萜、总皂苷、灵芝酸A、氨基酸、微量元素进行分析,并考察了不同品种灵芝提取物的1,1-二苯基-2-三硝基苯肼(DPPH)自由基、羟基自由基(·OH)的清除能力.结果表明,白肉灵芝的粗蛋白含量、氨基酸总量分别为...

Chemical Constituents and Antioxidant Activities in Vitroof Ganoderma leucocontextum

To study the main chemical components and antioxidant activity of Ganoderma leucocontextum,Ganoderma lucidum and Ganoderma sinense were used as control,a variety of analysis methods were used to analyze the nutritional components,polysaccharides,triterpenes,total saponins,ganoderic acid A,amino acids,and trace elements of G.leucocontextum.The scavenging activities of 1,1-diphenyl-2-trinitrophenylhydrazine(DPPH)free radical and hydroxyl radical(·OH)of G.leucocontextum extracts from different varieties were investigated.The results showed that the crude protein content and total amino acid content of G.leucocontextum were 18.02%~19.02%,113.61~163.51 mg/g,which were significantly higher than G.lucidum and G.sinense.The main activity contents of polysaccharides,triterpenoids and ganoderma acid A in G.leucocontextum were 0.99%~1.42%,1.22%~1.40%,0.069%~0.084%,respectively,which were significantly higher than in G.lucidum and G.sinense.The content of total amino acids in G.leucocontextum ranged from 113.61 mg/g to 163.51 mg/g,and the contents of glutamic acid and aspartic acid were high.IC50 value of methanol extract from G.leucocontextum on DPPH and hydroxyl free radical were 0.13~0.19 and 0.54~0.89 mg/mL,respectively,showing a good dose-response relationship,and its antioxidant activity was higher than that of G.sinense.This study provides a theoretical basis for the comprehensive development of G.leucocontextum.