| 杂质残留量对不同栽培方式下灵芝多糖肽定值的影响 |
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| 引用本文： | 林冬梅,王联福,罗虹建,杨宝学,林占熺,林树钱,王赛贞. 杂质残留量对不同栽培方式下灵芝多糖肽定值的影响[J]. 食品安全质量检测学报, 2022, 13(24): 8068-8074 |
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| 作者姓名： | 林冬梅 王联福 罗虹建 杨宝学 林占熺 林树钱 王赛贞 |
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| 作者单位： | 福建农林大学国家菌草工程技术研究中心,福建农林大学国家菌草工程技术研究中心,福建农林大学国家菌草工程技术研究中心,福建农林大学国家菌草工程技术研究中心,北京大学基础医学院药理学系,福建农林大学国家菌草工程技术研究中心,福建农林大学国家菌草工程技术研究中心 |
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| 基金项目： | 中央引导地方科技发展专项(2021L3008)、福建省重大专项(2021NZ0101)、学科交叉融合推动菌草科学及产业高质量发展项目(XKJC 71202103A) |
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| 摘 要： | 目的 依据国家标准样品定值要求,为提高标准样品纯度,降低杂质残留量,选择最佳栽培方式的灵芝子实体用于灵芝多糖肽(Ganoderma lucidum polysaccharide peptide, GL-PPSQ2)标准样品的研制。方法 基于高效液相色谱法(high performance liquid chromatography, HPLC),采用面积归一法对GL-PPSQ2主成分进行定量分析,卡尔费休库伦法(K-F)测定水分,热重分析法(thermogravimetricanalysis,TG)测定灰分,微波消解-电感耦合等离子体质谱法(inductively coupled plasma-mass spectrometry, ICP-MS)测定14种微量元素,顶空-气相色谱法(headspace-gas chromatography, HS-GC)测量溶剂残留;质量平衡法对菌草层架(A)、菌草覆土(B)、苡仁壳层架(C)、短段木覆土(D) 4组不同栽培方式所得GL-PPSQ2进行定值。结果 GL-PPSQ2定值依次...
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| 关 键 词： | 灵芝多糖肽 杂质残留量 定值 栽培方式 质量平衡法 |
| 收稿时间： | 2022-03-18 |
| 修稿时间： | 2022-09-21 |
Effects of residual impurities on certification of Ganoderma lucidum polysaccharide peptides under different cultivation methods |
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| LIN Dong-Mei,WANG Lian-Fu,LUO Hong-Jian,YANG Bao-Xue,LIN Zhan-Xi,LIN Shu-Qian,WANG Sai-Zhen. Effects of residual impurities on certification of Ganoderma lucidum polysaccharide peptides under different cultivation methods[J]. Journal of Food Safety & Quality, 2022, 13(24): 8068-8074 |
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| Authors: | LIN Dong-Mei WANG Lian-Fu LUO Hong-Jian YANG Bao-Xue LIN Zhan-Xi LIN Shu-Qian WANG Sai-Zhen |
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| Affiliation: | National Engineering Research Center of JUNCAO Technology Fujian Agriculture and Forestry University,National Engineering Research Center of JUNCAO Technology Fujian Agriculture and Forestry University,National Engineering Research Center of JUNCAO Technology Fujian Agriculture and Forestry University,National Engineering Research Center of JUNCAO Technology Fujian Agriculture and Forestry University,Department of Pharmacology, School of Basic Medical Sciences, Peking University,National Engineering Research Center of JUNCAO Technology Fujian Agriculture and Forestry University,National Engineering Research Center of JUNCAO Technology Fujian Agriculture and Forestry University |
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| Abstract: | Objective According to the certification of national standards, Ganoderma lucidum(G. lucidum) fruiting body under the best cultivation conditions was selected for preparation of G. lucidum polysaccharide peptide,in order to improve the purity and reduce the residual amount of impurities. Methods The main component purity values of G. lucidum polysaccharide peptide was determined by high performance liquid chromatographic(HPLC) area normalization method, moisture was measured by Karl Fischer(K-F) method, ash content were determined by thermogravimetric analysis(TGA),14 trace elements were measured by microwave digestion-inductively coupled plasma mass spectrometry(ICP-MS)and solvent residue was determined by headspace-gas chromatographic(GC); the purity of G. lucidum polysaccharide peptides under four different cultivation conditions was analyzed by mass balance method. Results The purity of G. lucidum polysaccharide peptide(%): adlay husk layer frame cultivation(97.40)>Juncao layer frame cultivation (96.50)>Short-segment wood covering soil cultivation (96.08)> Juncao covering soil cultivation (94.40); The yields (%) of G. lucidum polysaccharide peptides were as follows: Juncao cover soil(0.29)>Short section wood cover soil(0.18)>Juncao layer frame(0.15)>adlay husk layer frame(0.071). Conclusion According to the experimental results, short-segment wood covered with soil and Juncao layer frame cultivation should be selected as the raw material for preparation of G. lucidum polysaccharide peptide standard samples. |
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| Keywords: | G. lucidum polysaccharide peptides purity cultivation conditions residual impurities mass balance method |
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