富硒乳酸菌的筛选和体外活性的研究

为筛选富硒乳酸菌,利用其将无机硒转化为有机硒,用于开发富硒产品,实验以现存的15株乳酸菌为实验材料,将质量浓度为20 μg/mL的硒加入MRS培养基中,检测菌体OD值筛选富硒菌;通过测定生长曲线和检测培养基中硒含量不同时的菌体密度来确定富硒菌合适的加硒时间和富硒浓度;使用3,3'-二氨基联苯胺(3,3'-DAB)比色法...

Screening of Selenium-enriched Lactic Acid Bacteria and Evaluation of Their Bioactivities in Vitro

Selenium-enriched(Se-enriched)lactic acid bacteria(LAB)can convert inorganic Se into organic one to produce new products.In this paper,Se-resistant LAB were screened out from 15 LAB by adding Se with a mass concentration of 20μg/mL to MRS medium and detecting the OD value.The appropriate Se adding time of Se-enriched LAB was determined by growth curve,and concentration was determined by cell density with different Se contents in the culture medium.The Se accumulation amount of LAB was measured through 3,3’-diaminobenzidine(3,3’-DAB)method.The in vitro activity of Se-enriched LAB and its survival ability in human intestinal tract were evaluated by detecting the antioxidant activity,bile salt resistance and artificial gastric fluid resistance of bacteria.Results showed that 9 Se-enriched LAB were screened out from 15,the appropriate addition time was 2 h,concentration was 40μg/mL,and the strain JX5 with the highest Se conversion rate was 81.80%.9 strains had different scavenging effects on free radicals DPPH and ABTS after Se enrichment,and had different abilities to resist bile salts and artificial gastric fluid,and the overall in vitro activity showed an increasing trend,especially strain JX5,increased significantly in four indexes,which indicated that the transformation of inorganic Se into organic one by LAB can improve the in vitro activity of bacteria.The antioxidant capacity and bile salt tolerance of 9 strains were improved to some extent.