王浆酸对刀豆蛋白A诱导大鼠急性肝损伤的保护作用及机制研究

目的:研究王浆酸对刀豆蛋白A（concanavalin A，Con A）诱导大鼠急性肝损伤的保护作用及作用机制。方法:将SD大鼠随机分成阴性对照，模型对照，联苯双酯阳性对照组，王浆、酸低、中、高剂量组（30、60、120 mg/kg·bw）。研究王浆酸对大鼠肝功能的影响，结合肝脏的组织形态学变化及肝细胞凋亡，评价王浆酸对Con A诱导的大鼠急性肝损伤的保护作用。通过分析王浆酸对大鼠炎症、氧化应激、肝细胞周期以及脾脏和外周血T淋巴细胞分类的影响，研究其对急性肝损伤的保护作用机制。结果:与模型组比较，王浆酸高剂量组大鼠血清中谷草转氨酶、谷丙转氨酶、乳酸脱氢酶和碱性磷酸酶的酶活力极显著降低（P<0.01）；肝脏病理组织学观察，发现王浆酸各剂量组大鼠肝细胞损伤程度均有所改善；肝细胞的凋亡率极显著降低（P<0.01）。机制研究表明，王浆酸各剂量组大鼠血清中干扰素γ、肿瘤坏死因子α、白介素10、白介素6较模型组极显著降低（P<0.01），血清中丙二醛的含量极显著降低，超氧化物歧化酶、谷胱甘肽过氧化物酶活力极显著提高（P<0.01），王浆酸还可以极显著抑制肝细胞向G₂期增殖（P<0.01）且各剂量组大鼠脾脏及外周血中CD3+、CD4+和CD8+的比例显著提高（P<0.05）。结论:王浆酸对Con A诱导大鼠急性肝损伤有保护作用，机制可能与抑制炎症、抗氧化、提高机体免疫功能有关。

Protective Effect and Mechanism of Royal Jelly Acid on Liver InjuryInduced by Con A in Rats

Objective:To study the protective effect and mechanism of royal jelly acid on Con A-induced acute liver injury in rats.Methods:SD rats were randomly divided into negative control group,model control group,biphenyl diester positive control group,low,medium and high(30,60,120 mg/kg·bw)dose group.In order to evaluate the protective effect of royal jelly on acute liver injury induced by Con A,we studied the effects of royal jelly on liver function,combined with the changes of liver histomorphology and hepatocyte apoptosis.By analyzing the effects of royal jelly acid on inflammation,oxidative stress,hepatocyte cycle and T lymphocyte classification of spleen and peripheral blood in rats,the protective mechanism of royal jelly acid on acute liver injury was studied.Results:Compared with the model group,AST,ALT,LDH and AKP activities in the polar serum of the high-dose group significantly decreased(P<0.01);the degree of liver cell injury was improved;histopathological observation of liver showed that the groups in each dose of the royal jelly were significantly decreased(P<0.01);IL-6,IL^-10,TNF-αand INF-γin serum were significantly declined(P<0.01);the content of MDA in serum was significantly decreased and the activity of SOD and GSH-Px in serum significantly increased(P<0.01);the proliferation of hepatocytes to G2 phase was significantly inhibited in all dosage groups(P<0.05);apoptosis rate of hepatocytes significantly reduced(P<0.01);the ratio of CD3⁺,CD4⁺and CD8⁺in spleen and peripheral blood significantly increased in all dosage groups.Conclusion:Royal jelly acid has protective effect on acute liver injury induced by Con A in rats.The mechanism may be related to the inhibition of inflammation,antioxidation and improvement of immune function.