少花桂叶香气活性成分分析鉴定

对少花桂叶的香气活性成分进行分析鉴定.采用顶空固相微萃取制备样品,用两根色谱柱(极性柱DB-WAX和弱极性柱DB-5)进行气相色谱-质谱联用分析,并用DB-5柱进行直接强度法气相色谱—嗅闻分析(GC-O),基于保留指数、质谱库检索、气味特征鉴定化合物.结果:共鉴定出88种挥发性成分,含量高(＞1％)的成分为芳樟醇、香豆...

Analysis and Identification of Odor-active Compounds in Leaves of Cinnamomum pauciflorum Nees

The object of this study was to identify odor-active compounds in leaves of Cinnamomum pauciflorum Nees.Headspace solid phase micro extraction(HS-SPME)combined with gas chromatography-mass spectrometry(GC-MS)and gas chromatography-olfactometry(GC-O)was utilized,while GC-MS analysis involved both a polar column(DB-WAX)and a weak polar column(DB-5),and GC-O analysis adopted direct aroma intensity detection and a DB-5 column.The compounds were identified by search of Nist 2010 mass spectral library and comparison with the published retention indices and odor characteristics.In total,eighty-eight volatiles were identified in GC-MS analysis,where linalool,coumarin,δ-cadinene,santalene,α-copaene,styrene,β-santalene,trans-cinnamaldehyde,bornyl acetate,and 1,8-cineole showed high levels(>1%).However,just twenty three odor-active compounds were identified during GC-O analysis.According to the score of odor intensity(≥3),trans-cinnamaldehyde,linalool,4-terpineol,bornyl acetate,β-santalene,and ethyl cinnamate were considered to contribute highly to the overall aroma.The results could provide guidance for development and application of Cinnamomum pauciflorum Nees.