复合酶辅助超声波提取菊苣根总黄酮的工艺优化及其抗氧化活性

目的:菊苣根总黄酮提取工艺条件的优化，并探究其体外抗氧化能力。方法:在单因素实验基础上，选用Box-Behnken试验设计方法，建立以超声时间、液料比、酶解时间、超声功率和复合酶（纤维素酶与果胶酶）的用量为自变量，菊苣根总黄酮得率为因变量的二次回归模型。根据菊苣根总黄酮对ABTS自由基和DPPH自由基的清除效果来判断其体外抗氧化能力。结果:复合酶辅助超声波法提取菊苣根总黄酮的最佳工艺条件为:复合酶用量2.2%、液料比37:1 mL/g、酶解时间66 min、超声功率59 W、超声时间24 min，在此条件下总黄酮得率为5.43 ± 0.12 mg/g。当提取的总黄酮溶液浓度为0.1 mg/mL时，对DPPH、ABTS自由基的清除率分别为84.45%和98.18%，IC₅₀值分别为0.04和0.021 mg/mL。结论:本研究利用响应面法优化了菊苣根总黄酮的提取工艺，建立了总黄酮得率的模拟回归方程，可用于菊苣根总黄酮提取工艺的参数优化。菊苣根总黄酮有着较好的体外抗氧化活性，可用于食品添加剂和开发新的抗氧化药物。

Optimization of Extraction Technology and Antioxidant Activity of Total Flavonoids from Roots of Cichorium Intybus L.by Ultrasonic Assisted with Complex Enzyme

Objective:Optimization of extraction technology of total flavonoids from roots of Cichorium intybus L.,and to study its antioxidative activity in vitro.Methods:On the basis of single-factor test,Box-Behnken test design method was used to establish a quadratic regression model with ultrasonic time,liquid-solid ratio,enzymatic hydrolysis time,ultrasonic power and enzyme(cellulase and pectinase)dosage as independent variables,and the total flavonoids yield of chicory roots as the responsive values.The antioxidative activity in vitro of total flavonoids from chicory roots extracted under optimized conditions was evaluated by scavenging ABTS and DPPH free radicals.Results:The optimum extraction conditions of total flavonoids of chicory roots by ultrasound assisted with complex enzyme were as follows:enzyme dosage was 2.2%,liquid-Solid ratio was 37:1 mL/g,enzymatic hydrolysis time was 66 min,ultrasonic power was 59 W,and ultrasonic time was 24 min.Under these conditions,the total flavonoids yield was 5.43±0.12 mg/g.The scavenging rates of DPPH and ABTS radical were 84.45%and 98.18%respectively at the concentration of total flavonoids solution was 0.1 mg/mL,and IC50 were 0.04 and 0.021 mg/mL,respectively.Conclusion:In this study,the extraction process of total flavonoids from chicory roots was optimized by response surface method,and a simulated regression equation of total flavonoids yield was established,which could be used for parameter optimization of extraction process of total flavonoids from chicory roots.The total flavonoids of chicory roots had good antioxidant activity in vitro and could be used as food additives and to develop new antioxidant drugs.