| 三波长分光光度法测定芹菜叶中黄酮的含量 |
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| 引用本文： | 肖坤福,张春牛. 三波长分光光度法测定芹菜叶中黄酮的含量[J]. 食品研究与开发, 2005, 26(4): 134-136 |
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| 作者姓名： | 肖坤福 张春牛 |
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| 作者单位： | 浙江省丽水市丽水学院化学系,323000 |
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| 摘 要： | 采用三波长分光光度法测定芹菜叶中黄酮的含量，消除了由于芹菜叶中黄酮光谱吸收峰不对称给定量分析造成的影响。并校正了由于干扰组分的吸收光谱具有线性吸收产生的基线倾斜。实验结果回归方程为△A=0．0045C-0．0007相关系数r=0．9996；黄酮的浓度在30～300μg/mL范围内，分别在波长为λ1=460nm、λ2=420nm、λ3=380nm处测吸光度时，则△A与浓度C之间呈良好的线性关系，可按标准曲线法进行定量分析。本法的回收率为96．8％-105．9％，变异系数小于0．49％。方法的准确度与精密度均令人满意，而且操作简便易行。
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| 关 键 词： | 三波长分光光度法 芹菜 黄酮 光谱吸收 变异系数 |
| 收稿时间： | 2004-11-22 |
| 修稿时间： | 2004-11-22 |
DETERMINATION OF TOTAL FLAVONOIDS IN CELERY LEAVES BY THREE-WAVELENGTH SPECTROPHOTOMETRY |
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| XIAO Kunfu,ZHANG Chunniu. DETERMINATION OF TOTAL FLAVONOIDS IN CELERY LEAVES BY THREE-WAVELENGTH SPECTROPHOTOMETRY[J]. Food Research and Developent, 2005, 26(4): 134-136 |
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| Authors: | XIAO Kunfu ZHANG Chunniu |
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| Abstract: | Determination of flavonoids in celery leaves by three-wavelength spectrophotometry can eliminate the absorbance error of interfering components,turbid solution and the scattering effect. Back-ground changed with concentration changed and asymmetric absorption peak problems can be solved also.The method is more advantageous than tranditional spectrophotometry method.The recovery is 96.8%-105.9% and the coefficient of variation is less than 0.49%. |
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| Keywords: | flavonoids celery three-wavelength spectrophotometry |
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