TLC-FID assessment of lipid oxidation as applied to fish lipids rich in triglycerides

Changes in the polar lipid content of fatty fish oils were studied by quantitative thin layer chromatography-flame ionization detection (TLC-FID) during an accelerated oxidation test. There was a direct linear correlation between increase in polar lipids and increase in weight of the oil samples. Comparison of increases in polar lipids with thiobarbituric acid values gave a hyperbolic correlation curve. However, a linear correlation was obtained in a semilogarithmic system. The simple and time-saving TLC-FID method for the analysis of the polar lipid content of oils as compared to a column chromatographic method suggests that this new method could be used in quality control of edible oils. Presented in part at the ISF/AOCS World Congress, New York City, April 1980.