Application of thin-layer chromatography to the quantitative estimation of tissue triglycerides II. Influence of methyl parathion on the composition of liver triglycerides in the rat

Methyl parathion fed at 10 ppm in a high protein low fat diet inhibited 46.9% of the total liver carboxylesterase activity. The total fatty acid composition of liver triglycerides was not significantly altered. However, the methyl parathion-fed rats showed a higher percentage of saturated acids in the 2-position of the glyceride molecule. Triglyceride analysis employing the multiple TLC-GLC technique (5) also showed a slightly higher percentage of saturated glycerides and those containing 1 and 2 double bonds than those in the control group. Triglyceride patterns in both groups were in general agreement with those calculated from the fatty acid distribution as suggested by Vander Wal (11). Presented at the AOCS meeting in Houston, Texas, April, 1965.