农药多残留分析中QuEchERS方法介绍

主要讨论在农药多残留分析中的一种简便、快速、安全、价格低廉的分析方法——QuEchERS法。该方法的主要步骤是用含1%醋酸的乙腈对样品进行浸提,再加入无水硫酸镁与醋酸钠振荡促使其分层,随后进行分散固相萃取,即将浸提液转移至含有PSA吸附剂、硫酸镁的离心管中,运用Teflon涂层离心管进行离心,取离心液至自动进样瓶用于GC/MS或LC/MS进行测定,并对其稳定性和可靠性进行了分析和评价。同时本方法也可根据可供选择的分析仪器种类、检测限、靶标农药的范围以及使用介质的差异进行适当调整。

Introduce of Quick, Easy, Cheap, Effective, Rugged and Safe (QuEchERS) Approach for Determining Pesticide Residues

This chapter describes a simple, fast, and inexpensive method for the determination of pesticides in foods and potentially other matrices. The method, known as the quick, easy, cheap, effective, rugged, and safe (QuEchERS) method for pesticide residues involves the extraction of the sample with acetonitrile (MeCN) containing 1% acetic acid (Hac) and simultaneous liquid-liquid partitioning formed by adding anhydrous magnesium sulfate (MgSO4) plus sodium acetate (NaAc) following by a simple cleanup step known as dipersive solid-phase extraction (SPE). The QuEchERS method is carried out by shaking a Teflon centrifuge tube which contains 1mL of 1% Hac in MeCN plus 0.4g anh. MgSO4 and 0.1 g anh NaAc per sample. The tube is then centrifuged, and a partion of the extract is transferred to a tube containing 50 mg primary secondary amine (PSA) sorbent plus 150 mg anh. MgSO4 per mL extract (the dispersive-SPE cleanup step). Then, the extract is transferred to autosampler vials for concurrent analysis by GC/MS and LC/MS-MS. Estimation of the method’ stability and reliability and its use was done too. Alternatives was adjusted by optional instruments, LOQ, analytical scope and different matrices.