| 分光光度法测定破布叶中总黄酮 |
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| 引用本文： | 毕和平,韩长日,王芳,乔丽艳,陈光英. 分光光度法测定破布叶中总黄酮[J]. 广东化工, 2006, 33(3): 43-45 |
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| 作者姓名： | 毕和平 韩长日 王芳 乔丽艳 陈光英 |
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| 作者单位： | 海南师范大学,化学系,海南,海口,571158;海南师范大学,生物系,海南,海口,571158 |
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| 摘 要： | 利用分光光度法测定了破布叶的叶、茎、果中的总黄酮含量,结果表明,破布叶叶、茎、果中的总黄酮质量分数分别为16．94％、5．15％和1．52％。回归方程为A=11．4486C-0．00543,r=0．9996。叶、茎、果的平均加标回收率分别为102．3％、97．6％和92．6％,RSD依次为1．01％、1．94％和2．76％(n=5)。本法操作简便、可行、重现性好。
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| 关 键 词： | 破布叶 总黄酮 分光光度法 含量测定 |
| 收稿时间： | 2006-02-18 |
| 修稿时间： | 2006-02-18 |
Spectrophotometric Determination of the Total Flavonoids in Microcos Paniculata L. |
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| Bi Heping,Han Changri,Wang fang,Qiao Liyan,Chen guangying. Spectrophotometric Determination of the Total Flavonoids in Microcos Paniculata L.[J]. Guangdong Chemical Industry, 2006, 33(3): 43-45 |
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| Authors: | Bi Heping Han Changri Wang fang Qiao Liyan Chen guangying |
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| Affiliation: | 1. Chemical Department, Hainan Normal University, Haikou 5711583 2. Biological Department, Hainan Normal University, Haikou 571158, China |
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| Abstract: | The total flavonoids in leaf, stem and fruit of Microcos Paniculata L were determined by spectrophotometry at 500 nm. The linear regression equation is A =11.448 6C - 0.00543, r = 0.9996. Results was 16.94% in leaf, the average recovery was 102.3%, RSD=1.82% (n=5); 5.15% in stem, the average recovery was 98.4 %, RSD =1.95%(n = 5); and 1.52 % in fruit, the average recovery was 94.0 %, RSD = 2.55 %(n = 5). The method is simple, reproducible and practicable for the determination of total flavones. |
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| Keywords: | Microcos Paniculata L flavonoids spectrophotometry quantitative analysis |
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