| 葡萄籽白藜芦醇的纯化及体外抗氧化功能的研究 |
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| 引用本文： | 冯涛,董凌杰,杜宁. 葡萄籽白藜芦醇的纯化及体外抗氧化功能的研究[J]. 食品工业, 2010, 0(2) |
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| 作者姓名： | 冯涛 董凌杰 杜宁 |
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| 作者单位： | 上海应用技术学院香料香精技术与工程学院;金坛食品药品监督管理局; |
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| 基金项目： | 上海高校选拔培养优秀青年教师科研专项基金.项目编号:yyy-07021 |
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| 摘 要： | 选用葡萄酿酒下脚料—葡萄籽为原料,乙醇为提取溶剂,对原料进行提取后纯化粗提液中的白藜芦醇,测定其体外抗氧化活性。比较了大孔树脂法及双水相萃取法对白藜芦醇的纯化效果,并以DPPH法研究该纯化产品的体外抗氧化活性。通过不同树脂对于白藜芦醇吸附率及解吸率的比较,不同浓度乙醇洗脱率的比较,得出:选用AB-8大孔树脂,乙醇洗脱浓度90%。在此最佳洗脱条件下对白藜芦醇的粗品进行动态吸附和洗脱,得到纯化产品,纯度达到11.8%。对最终产物进行DPPH体外抗氧化活性作用试验,发现随着浓度的增大,白藜芦醇对于DPPH的清除率也随之提高,浓度为15μg/mL的样品的清除率可达到60.98%。
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| 关 键 词： | 葡萄籽 白藜芦醇 大孔树脂 双水相萃取 体外抗氧化活性 |
Study on Purification of Grape Seed Resveratrol and its Antioxidative Function in Vitro |
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| Feng Tao ,Dong Ling-jie ,Du Ning .School of Perfume , Aroma Technology of. Study on Purification of Grape Seed Resveratrol and its Antioxidative Function in Vitro[J]. The Food Industry, 2010, 0(2) |
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| Authors: | Feng Tao Dong Ling-jie Du Ning .School of Perfume Aroma Technology of |
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| Affiliation: | Feng Tao 1,Dong Ling-jie 1,Du Ning 2 1.School of Perfume , Aroma Technology of Shanghai Institute of Technology(Shanghai 200235),2.Food&Drug Supervision , Administration Bureau of Jintan(Changzhou 213200) |
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| Abstract: | The main topic of this experiment is to use the remainder of wine product as the raw material.And the ethanol was used as extraction solvent.After the initial extraction and enrichment to the raw material, antioxidability in vitro was detected with the extraction solution.The purpose of the experiment is to compare the resveratrol purification effect of macroporous resin or the aqueous two-phase extraction method,and to detect the antioxidability in vitro of the purified resveratrol.Relative resveratrol con... |
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| Keywords: | grape seed resveratrol macroporous resin aqueous two-phase extraction in vitro antioxidability |
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